Document Detail


Raphanus sativus and its isothiocyanates inhibit vascular smooth muscle cells proliferation and induce G(1) cell cycle arrest.
MedLine Citation:
PMID:  16546717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mu (Raphanus sativus, Korean White Radish) crude extract (Mu-CE) has been studied for its anti-proliferative activity on mouse aortic smooth muscle cells. The abnormal growth of vascular smooth muscle cells (VSMC) is a prominent feature of vascular disease, including atherosclerosis, restenosis after angioplasty. We examined the mechanisms of the action of Mu-CE on VSMC proliferation. The viability of VSMC decreased to 35% at 24 h of treatment with Mu-CE. Treatment of Mu-CE showed potent inhibitory effects on the DNA synthesis of cultured VSMC. In addition, Mu-CE induced apoptosis using cell death ELISA assay. These inhibitory effects were associated with G1 cell cycle arrest. Treatment of Mu-CE, which induced a cell-cycle arrest in G1-phase, induced down-regulation of cyclins and CDKs and up-regulation of the CDK inhibitor p21 expression, whereas up-regulation of p27 by Mu-CE was not observed. Then, total isothiocyanates (ITC) including four different 4-(Methylthio)-3-butenyl isothiocyanate (MTBITC), allyl isothiocayanate (AITC), benzyl isothiocyanate (BITC), and phenethyl isothiocyanate (PEITC) was isolated from n-hexane extracts of Mu. When the VSMC were treated with ITC, the cell viability was significantly decreased. These findings indicate the efficacy of Mu-CE in inhibiting cell proliferation, G1- to S-phase cell-cycle progress on VSMC.
Authors:
Seok-Jong Suh; Sung-Kwon Moon; Cheorl-Ho Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-12-09
Journal Detail:
Title:  International immunopharmacology     Volume:  6     ISSN:  1567-5769     ISO Abbreviation:  Int. Immunopharmacol.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-03-20     Completed Date:  2006-06-01     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100965259     Medline TA:  Int Immunopharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  854-61     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Dongguk University College of Oriental Medicine and National Research Laboratory for Glycobiology, Sukjang-Dong 707, Kyungju, Kyungbuk 780-714, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta
Apoptosis
Cell Line
Cell Proliferation / drug effects
Cyclin D1 / antagonists & inhibitors,  metabolism
Cyclin E / antagonists & inhibitors,  metabolism
Cyclin-Dependent Kinase 4 / metabolism
Cyclin-Dependent Kinase Inhibitor p21 / metabolism
G1 Phase / drug effects
Isothiocyanates / isolation & purification,  pharmacology*
Mice
Muscle, Smooth, Vascular / cytology
Myocytes, Smooth Muscle / cytology,  drug effects*
Plant Extracts / pharmacology
Raphanus / chemistry*
Chemical
Reg. No./Substance:
0/Cyclin E; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Isothiocyanates; 0/Plant Extracts; 136601-57-5/Cyclin D1; EC 2.7.11.22/Cyclin-Dependent Kinase 4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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