Document Detail


Rapamycin and curcumin induce apoptosis in primary resting B chronic lymphocytic leukemia cells.
MedLine Citation:
PMID:  19373661     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
B chronic lymphocytic leukemia (B-CLL) cells exist in patients as slowly accumulating resting as well as proliferating B cells. In this study, we examined whether Rapamycin and Curcumin, two naturally occurring compounds shown to have apoptotic effects, could selectively induce apoptosis in resting B-CLL cells. Mononuclear cells isolated from patients with B-CLL were treated with these agents and analysed by AnnexinV/propidium iodide binding, caspase activity, and changes in bcl-2/Bax ratio. Rapamycin and curcumin significantly induced apoptosis in resting B-CLL cells obtained from patients with CLL. Furthermore, rapamycin and curcumin increased caspase 9, 3 and 7 activity, decreased anti-apoptotic bcl-2 levels, and increased the pro-apoptotic protein Bax. These data suggest rapamycin and curcumin may be an effective treatment for B-CLL and are of high clinical significance considering the growing population of patients and lack of efficient treatment for this malignant disease.
Authors:
Rami Hayun; Eitan Okun; Alain Berrebi; Lev Shvidel; Lucette Bassous; Benjamin Sredni; Uri Nir
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Leukemia & lymphoma     Volume:  50     ISSN:  1029-2403     ISO Abbreviation:  Leuk. Lymphoma     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-17     Completed Date:  2009-09-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9007422     Medline TA:  Leuk Lymphoma     Country:  England    
Other Details:
Languages:  eng     Pagination:  625-32     Citation Subset:  IM    
Affiliation:
The Mina and Everard Goodman Faculty of Life Sciences, Safdie Institute for AIDS and Immunology Research, Bar-Ilan University, Ramat-Gan, Israel.
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Antineoplastic Agents / pharmacology
Apoptosis / drug effects*
Blotting, Western
Caspase 3 / metabolism
Caspase 7 / metabolism
Caspase 9 / metabolism
Curcumin / pharmacology*
Female
Flow Cytometry
Humans
Immunosuppressive Agents / pharmacology
Leukemia, Lymphocytic, Chronic, B-Cell / blood*,  metabolism,  pathology
Leukocytes, Mononuclear / drug effects*,  metabolism,  pathology
Male
Middle Aged
Proto-Oncogene Proteins c-bcl-2 / metabolism
Sirolimus / pharmacology*
Time Factors
Tumor Cells, Cultured
bcl-2-Associated X Protein / metabolism
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Immunosuppressive Agents; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 458-37-7/Curcumin; 53123-88-9/Sirolimus; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 7; EC 3.4.22.-/Caspase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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