| Random mutagenesis reveals a region important for gating of the yeast K+ channel Ykc1. | |
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MedLine Citation:
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PMID: 9305624 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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YKC1 (TOK1, DUK1, YORK) encodes the outwardly rectifying K+ channel of the yeast plasma membrane. Non-targeted mutations of YKC1 were isolated by their ability to completely block proliferation when expressed in yeast. All such mutations examined occurred near the cytoplasmic ends of the transmembrane segments following either of the duplicated P loops, which we termed the 'post-P loop' (PP) regions. These PP mutations specifically caused marked defects in the 'C1' states, a set of interrelated closed states that Ykc1 enters and exits at rates of tens to hundreds of milliseconds. These results indicate that the Ykc1 PP region plays a role in determining closed state conformations and that non-targeted mutagenesis and microbial selection can be a valuable tool for probing structure-function relationships of ion channels. |
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Authors:
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S H Loukin; B Vaillant; X L Zhou; E P Spalding; C Kung; Y Saimi |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The EMBO journal Volume: 16 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 1997 Aug |
Date Detail:
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Created Date: 1997-10-17 Completed Date: 1997-10-17 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 4817-25 Citation Subset: IM; S |
Affiliation:
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Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Fungal Proteins / chemistry, genetics, metabolism* Ion Channel Gating* Molecular Sequence Data Mutagenesis* Oocytes Patch-Clamp Techniques Plasmids / genetics Potassium Channels / chemistry, genetics, metabolism* Saccharomyces cerevisiae / genetics*, growth & development, metabolism Saccharomyces cerevisiae Proteins* Sequence Alignment Sequence Analysis, DNA Structure-Activity Relationship Transfection Xenopus |
| Grant Support | |
ID/Acronym/Agency:
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36386//PHS HHS; GM 22714/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 0/Potassium Channels; 0/Saccharomyces cerevisiae Proteins; 0/TOK1 protein, S cerevisiae |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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