Document Detail


Random mutagenesis reveals a region important for gating of the yeast K+ channel Ykc1.
MedLine Citation:
PMID:  9305624     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
YKC1 (TOK1, DUK1, YORK) encodes the outwardly rectifying K+ channel of the yeast plasma membrane. Non-targeted mutations of YKC1 were isolated by their ability to completely block proliferation when expressed in yeast. All such mutations examined occurred near the cytoplasmic ends of the transmembrane segments following either of the duplicated P loops, which we termed the 'post-P loop' (PP) regions. These PP mutations specifically caused marked defects in the 'C1' states, a set of interrelated closed states that Ykc1 enters and exits at rates of tens to hundreds of milliseconds. These results indicate that the Ykc1 PP region plays a role in determining closed state conformations and that non-targeted mutagenesis and microbial selection can be a valuable tool for probing structure-function relationships of ion channels.
Authors:
S H Loukin; B Vaillant; X L Zhou; E P Spalding; C Kung; Y Saimi
Related Documents :
11707264 - Functional identification of sterol-4alpha-methyl oxidase cdnas from arabidopsis thalia...
1988444 - Yeast thioredoxin genes.
7828874 - Yke2, a yeast nuclear gene encoding a protein showing homology to mouse ke2 and contain...
6321234 - Nucleotide sequence of a yeast trnaarg3a gene and its transcription in a homologous in ...
24643174 - Human papillomavirus genomics: past, present and future.
1971514 - Isolation of a cdna encoding human rev-erba alpha: transcription from the noncoding dna...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The EMBO journal     Volume:  16     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-10-17     Completed Date:  1997-10-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4817-25     Citation Subset:  IM; S    
Affiliation:
Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Fungal Proteins / chemistry,  genetics,  metabolism*
Ion Channel Gating*
Molecular Sequence Data
Mutagenesis*
Oocytes
Patch-Clamp Techniques
Plasmids / genetics
Potassium Channels / chemistry,  genetics,  metabolism*
Saccharomyces cerevisiae / genetics*,  growth & development,  metabolism
Saccharomyces cerevisiae Proteins*
Sequence Alignment
Sequence Analysis, DNA
Structure-Activity Relationship
Transfection
Xenopus
Grant Support
ID/Acronym/Agency:
36386//PHS HHS; GM 22714/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fungal Proteins; 0/Potassium Channels; 0/Saccharomyces cerevisiae Proteins; 0/TOK1 protein, S cerevisiae
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants.
Next Document:  In vivo disassembly of free polyubiquitin chains by yeast Ubp14 modulates rates of protein degradati...