Document Detail

Random UV Mutagenesis Approach for Enhanced Biodegradation of Sulfonated Azo Dye, Green HE4B.
MedLine Citation:
PMID:  23315264     Owner:  NLM     Status:  Publisher    
The objective of the study was to execute mutant bacteria for efficient biodegradation of sulfonated azo dye, Green HE4B (GHE4B). UV irradiation was used to introduce random mutations in Pseudomonas sp. LBC1. Genetic alterations induced by UV irradiation in selected mutant bacteria were confirmed by random amplification of polymorphic DNA technique. The mutant bacteria named as Pseudomonas sp. 1 F reduced the time required for complete degradation of recalcitrant dye GHE4B by 25 % when compared with the wild one. The biodegradation was monitored by UV-Vis spectrophotometric analysis. Activities of enzymes like laccase, lignin peroxidase, veratryl alcohol oxidase, and NADH dichlorophenol indophenol reductase were found to be boosted in mutant bacteria as a consequence of UV-induced mutation. Matrix-assisted laser desorption/ionization-time of flight analysis of differentially expressed proteins of mutant bacteria suggested active role of antioxidant enzymes in the degradation of the dye. The degradation product was analyzed by Fourier transform infrared spectroscopy, high-performance thin-layer chromatography, and gas chromatography-mass spectrometry. Results revealed few variations in the degradation end products of wild-type and mutant bacteria. Phytotoxicity study underlined the safer biodegradation of GHE4B by mutant Pseudomonas sp. 1 F.
Swati M Joshi; Shrirang A Inamdar; Jyoti P Jadhav; Sanjay P Govindwar
Related Documents :
24301364 - Aldrin epoxidase activity and cytochrome p-450 content of sawfly larvae,pergagrapta pol...
23359084 - Functional food quality of curcuma caesia, curcuma zedoaria and curcuma aeruginosa ende...
24307994 - Enzymes inhibition and antidiabetic effect of isolated constituents from dillenia indica.
24156774 - Identification of a suitable and selective inhibitor towards aldehyde oxidase catalyzed...
19567234 - Gamma-glutamylcysteine synthetase from plasmodium berghei.
18611114 - High-throughput enzymology and combinatorial mutagenesis for mining cytochrome p450 fun...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-15
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  -     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Biotechnology, Shivaji University, Kolhapur, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A closed system supports the developmental competence of human embryos after vitrification : Closed ...
Next Document:  Effects of menopausal hormone therapy on ductal carcinoma in situ of the breast.