Document Detail


Raman spectroscopy of single human tumour cells exposed to ionizing radiation in vitro.
MedLine Citation:
PMID:  21119222     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
This work investigates the capability of Raman spectroscopy (RS) to study the effects of ionizing radiation on single human tumour cells. Prostate tumour cells (cell line DU145) are cultured in vitro and irradiated to doses between 15 and 50 Gy with single fractions of 6 MV photons. Single-cell Raman spectra are acquired from irradiated and unirradiated cultures up to 5 days post-irradiation. Principal component analysis is used to distinguish the uniquely radiation-induced spectral changes from inherent sources of spectral variability arising from cell cycle differences and other known factors. We observe uniquely radiation-induced spectral changes which are correlated with both the irradiated dose and the incubation time post-irradiation. The spectral changes induced by radiation arise from biochemical differences in lipids, nucleic acids, amino acids and conformational protein structures between irradiated and unirradiated cells. To our knowledge, this study is the first use of RS to observe radiation-induced biochemical differences in single cells, and is the first use of vibrational spectroscopy to observe uniquely radiation-induced biochemical differences in single cells independent of concurrent cell-cycle- or cell-death-related processes.
Authors:
Q Matthews; Ag Brolo; J Lum; X Duan; A Jirasek
Publication Detail:
Type:  Journal Article     Date:  2010-11-30
Journal Detail:
Title:  Physics in medicine and biology     Volume:  56     ISSN:  1361-6560     ISO Abbreviation:  Phys Med Biol     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401220     Medline TA:  Phys Med Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  19-38     Citation Subset:  IM    
Affiliation:
Department of Physics and Astronomy, University of Victoria, Victoria BC V8W 3P6, Canada.
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