Document Detail


Raman spectroscopic characterization of structural changes in heated whey protein isolate upon soluble complex formation with pectin at near neutral pH.
MedLine Citation:
PMID:  23134232     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The mechanism leading to an alteration of heat aggregation of whey protein isolate (WPI) in the presence of pectin was investigated by assessing structural changes of proteins using Raman spectroscopy. WPI solutions were heated without or with pectin at 0.015-0.2 pectin to WPI weight ratios and pH 6.0 to 6.4. In the absence of pectin, thermal denaturation resulted in a loss of α-helical structure and an increase in β-structure and random coils of protein. At pH 6.0 and 6.2, heat aggregation of WPI was suppressed when pectin (0.05-0.15 pectin to WPI ratios) was present as shown by a decrease in turbidity and particle size. Concomitantly, changes in the secondary structures were reduced, indicating the enhanced stability of protein structure by pectin. Raman results also revealed that α-helix and β-sheet are dominant structures in heated WPI-pectin soluble complex and hydrogen bonding between biopolymers increased. The effect of pectin was pH dependent, indicating the involvement of electrostatic interaction.
Authors:
Sha Zhang; Zhong Zhang; Mengshi Lin; Bongkosh Vardhanabhuti
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-7
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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