Document Detail

Raman spectroscopic characterization of necrotic cell death.
MedLine Citation:
PMID:  19021382     Owner:  NLM     Status:  MEDLINE    
Raman spectroscopy has been used to estimate the biochemical changes due to necrosis in an in vitro model system comprised of a human malignant melanoma cell line (MEL-28). Combined oxygen and glucose deprivation was used to simulate necrotic cell death in tumors. Raman spectroscopy measurements of nonproliferating live cells and dead cells were made at 24, 48, and 72 hours. Quantitative estimates of the biochemical composition of live and dead cells were made by fitting cell spectra to the basis spectra of protein, lipid, RNA, DNA, and glycogen. A decrease in the relative amount of lipid and RNA, and an increase in the relative protein content, were observed in dead cells. A comparison of the spectra indicated the existence of conformational changes in protein and nucleic acids in dead cells. These results suggest that Raman spectroscopy could be used to detect necrotic cell death in tumors.
Nagapratima Kunapareddy; James P Freyer; Judith R Mourant
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  13     ISSN:  1083-3668     ISO Abbreviation:  J Biomed Opt     Publication Date:    2008 Sep-Oct
Date Detail:
Created Date:  2008-11-21     Completed Date:  2009-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  054002     Citation Subset:  IM    
Los Alamos National Laboratory, Bioscience Division, MS E535, Los Alamos, New Mexico 87545, USA.
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MeSH Terms
Cell Line, Tumor
DNA, Neoplasm / analysis*
Equipment Design
Equipment Failure Analysis
Lipids / analysis*
Melanoma / metabolism*,  pathology*
Neoplasm Proteins / analysis*
RNA, Neoplasm / analysis*
Spectrum Analysis, Raman / instrumentation*,  methods*
Grant Support
Reg. No./Substance:
0/DNA, Neoplasm; 0/Lipids; 0/Neoplasm Proteins; 0/RNA, Neoplasm

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