Document Detail


Raman and infrared microspectral imaging of mitotic cells.
MedLine Citation:
PMID:  16454901     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report the first ever Raman and infrared microspectroscopic images of human cells at different stages of mitosis. These spectroscopic methods monitor the distribution of condensed nuclear chromatin, and other biochemical components, utilizing inherent protein and DNA spectral markers, and, therefore, do not require the use of any stains. In conjunction with previously reported data from the G1, S, and G2 phases of the cell cycle, the complete cell division cycle has now been mapped by spectroscopic methods. Although the results reported here do not offer new insights into the distribution of biochemical components during mitosis, the recognition of cell division without the use of stains, and the possibility of doing so on living cells, may be useful for an automatic, spectroscopic determination of the proliferation rates of cells and tissues. Spectral images were constructed by plotting spectral intensities of DNA or protein versus the coordinates from which spectra were recorded. We found that both Raman and infrared intensities depend on the overall chromatin density variation among the individual subphases of mitosis.
Authors:
Christian Matthäus; Susie Boydston-White; Milos Miljković; Melissa Romeo; Max Diem
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Applied spectroscopy     Volume:  60     ISSN:  0003-7028     ISO Abbreviation:  Appl Spectrosc     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-02-03     Completed Date:  2006-02-28     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  0372406     Medline TA:  Appl Spectrosc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle Proteins / metabolism*
Cell Nucleus / metabolism*,  ultrastructure*
DNA / metabolism
HeLa Cells
Humans
Image Interpretation, Computer-Assisted / methods*
Mitosis / physiology*
Spectroscopy, Fourier Transform Infrared / methods*
Spectrum Analysis, Raman / methods*
Grant Support
ID/Acronym/Agency:
CA 090346/CA/NCI NIH HHS; GM 60654/GM/NIGMS NIH HHS; R01 CA090346/CA/NCI NIH HHS; R01 CA090346-04/CA/NCI NIH HHS; RR-03037/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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