Document Detail

Ragweed pollen extract intensifies lipopolysaccharide-induced priming of NLRP3 inflammasome in human macrophages.
MedLine Citation:
PMID:  23278511     Owner:  NLM     Status:  MEDLINE    
Ragweed pollen extract (RWE) possesses intrinsic NADPH oxidase activity that induces oxidative stress by initiating the production of intracellular reactive oxygen species (ROS). The ROS are important contributors to the manifestation of allergic inflammation; furthermore, concomitant exposure to an allergen and an endotoxin trigger a stronger inflammatory response. One of the main pro-inflammatory cytokines produced in inflammatory responses is interleukin-1β (IL-1β), and its production is associated with caspase-1-containing inflammasome complexes. Intracellular ROS have been implicated in NLRP3 inflammasome-mediated IL-1β production, therefore, we aimed to study whether RWE influences the function of NLRP3 inflammasome. Here we describe that, in the presence of NADPH, RWE significantly elevates lipopolysaccharide-induced IL-1β production of THP-1 cells as well as human primary macrophages and dendritic cells. We also demonstrate that increased IL-1β production is mediated through NLRP3 inflammasome in THP-1 macrophages. We provide evidence that RWE elevates cytosolic ROS level in these cells, and ROS inhibitors abolish IL-1β production. Furthermore, we show that RWE enhances lipopolysaccharide-induced gene transcription/expression of pro-IL-1β and key components of the inflammasome via a ROS-dependent mechanism.
Aliz Varga; Marietta M Budai; Sándor Milesz; Attila Bácsi; József Tőzsér; Szilvia Benkő
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  138     ISSN:  1365-2567     ISO Abbreviation:  Immunology     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-13     Completed Date:  2013-04-26     Revised Date:  2014-04-01    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  392-401     Citation Subset:  IM    
Copyright Information:
© 2012 University of Debrecen Immunology © 2012 Blackwell Publishing Ltd.
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MeSH Terms
Antigens, Plant / immunology,  pharmacology,  therapeutic use*
Carrier Proteins / genetics,  immunology*
Caspase 1 / genetics,  immunology
Cell Line
Free Radical Scavengers / pharmacology
Inflammasomes / drug effects*,  genetics,  immunology
Inflammation / immunology,  pathology
Interleukin-1beta / genetics,  immunology*
Lipopolysaccharides / pharmacology
Macrophage Activation / drug effects
Macrophages / cytology,  drug effects*,  immunology
NADP / metabolism,  pharmacology
Plant Extracts / immunology,  pharmacology*
Reactive Oxygen Species / antagonists & inhibitors
Transcription, Genetic / drug effects
Reg. No./Substance:
0/Antigens, Plant; 0/Carrier Proteins; 0/Free Radical Scavengers; 0/Inflammasomes; 0/Interleukin-1beta; 0/Lipopolysaccharides; 0/NLRP3 protein, human; 0/Plant Extracts; 0/Reactive Oxygen Species; 0/ragweed pollen; 53-59-8/NADP; EC 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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