| Radon-induced reduced apoptosis in human bronchial epithelial cells with knockdown of mitochondria DNA. | |
| | |
MedLine Citation:
|
PMID: 22891884 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Radon and radon progeny inhalation exposure are recognized to induce lung cancer. To explore the role of mitochondria in radon-induced carcinogenesis in humans, an in vitro partially depleted mitochondrial DNA (mtDNA) cell line (ρ-) was generated by treatment of human bronchial epithelial (HBE) cells (ρ+) with ethidium bromide (EB). The characterization of ρ- cells indicated the presence of dysfunctional mitochondria and might thus serve a reliable model to investigate the role of mitochondria. In a gas inhalation chamber, ρ- and ρ+ cells were exposed to radon gas produced by a radium source. Results showed that apoptosis was significantly increased both in ρ- and ρ+ cells irradiated by radon. Moreover, apoptosis in ρ- cells showed a lower level than in ρ+ cells. Radon was further found to depress mitochondrial membrane potential (MMP) of HBE cells with knockdown mtDNA. Production of reactive oxygen species (ROS) was markedly elevated both in ρ- and ρ+ cells exposed to radon. The distribution of phases of cell cycle was different in ρ- compared to ρ+ cells. Radon irradiation induced a rise in G2/M and decrease in S phase in ρ+ cells. In ρ- cells, G1, G2/M, and S populations remained similar to cells exposed to radon. In conclusion, radon-induced changes in ROS generation, MMP and cell cycle are all attributed to reduction of apoptosis, which may trigger and promote cell transformation, leading to carcinogenesis. Our study indicates that the use of the ρ- knockdown mtDNA HBE cells may serve as a reliable model to study the role played by mitochondria in carcinogenic diseases. |
| | |
Authors:
|
Bing-Yan Li; Jing Sun; Hong Wei; Yu-Zhi Cheng; Lian Xue; Zhi-Hai Cheng; Jian-Mei Wan; Ai-Qing Wang; Tom K Hei; Jian Tong |
Related Documents
:
|
7216904 - Ultrastructural and morphometric study of the langerhans cell in the normal human exoce... 6609204 - Neoplastic cells obtained from hodgkin's disease function as accessory cells for mitoge... 15166504 - Differentiation of hidroacanthoma simplex from clonal seborrheic keratosis--an immunohi... 7286354 - Electron microscopic investigation on the local cellular reaction to primary malignant ... 23144804 - Scribble acts in the drosophila fat-hippo pathway to regulate warts activity. 6737314 - Membrane protein differences correlated with the development of mating competence in te... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of toxicology and environmental health. Part A Volume: 75 ISSN: 1528-7394 ISO Abbreviation: J. Toxicol. Environ. Health Part A Publication Date: 2012 |
Date Detail:
|
Created Date: 2012-08-15 Completed Date: 2012-10-22 Revised Date: 2013-04-18 |
Medline Journal Info:
|
Nlm Unique ID: 100960995 Medline TA: J Toxicol Environ Health A Country: England |
Other Details:
|
Languages: eng Pagination: 1111-9 Citation Subset: IM |
Affiliation:
|
Department of Toxicology, School of Public Health, Soochow University, Suzhou, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects,
radiation effects* Bronchi / drug effects, metabolism, radiation effects* Carcinogens, Environmental / toxicity* Cell Cycle / radiation effects Cell Line Cell Transformation, Neoplastic / radiation effects DNA, Mitochondrial / antagonists & inhibitors*, metabolism Ethidium / pharmacology Gene Knockdown Techniques Humans Intercalating Agents / pharmacology Membrane Potential, Mitochondrial / radiation effects Mitochondria / drug effects, metabolism, radiation effects* Radon / toxicity* Radon Daughters / toxicity Reactive Oxygen Species / metabolism Respiratory Mucosa / drug effects, metabolism, radiation effects* Toxicity Tests, Chronic / methods |
| Grant Support | |
ID/Acronym/Agency:
|
CA49062/CA/NCI NIH HHS; ES05786/ES/NIEHS NIH HHS; P01 CA049062/CA/NCI NIH HHS; P42 ES010349/ES/NIEHS NIH HHS; P42 ES10349/ES/NIEHS NIH HHS; R01 ES005786/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Carcinogens, Environmental; 0/DNA, Mitochondrial; 0/Intercalating Agents; 0/Radon Daughters; 0/Reactive Oxygen Species; 10043-92-2/Radon; 3546-21-2/Ethidium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Graphene Film Growth on Polycrystalline Metals.
Next Document: Effects of 1800-MHz Radiofrequency Fields on Circadian Rhythm of Plasma Melatonin and Testosterone i...