Document Detail


Radiosensitization in human breast carcinoma cells by thymoquinone: role of cell cycle and apoptosis.
MedLine Citation:
PMID:  21557727     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
TQ (thymoquinone), the bioactive constituent of black seed (Nigella sativa), has been shown to inhibit the growth of various human cancers both in vitro and in vivo. This study reports the radiosensitizing effect of TQ on human breast carcinoma cells (MCF7 and T47D). TQ in combination with single dose of ionizing radiation (2.5 Gy) was found to exert supra-additive cytotoxic effects on both the carcinomas as measured by cell proliferation and colony-formation assays. Annexin V binding and FACS analysis revealed the role of enhanced apoptosis and cell cycle modulation in the mechanism of TQ-mediated radiosensitization, thus supporting TQ as an adjuvant for preclinical testing in cancer chemo-radiotherapy.
Authors:
Reelma Velho-Pereira; Amit Kumar; Badri N Pandey; Aarti G Jagtap; Kaushala P Mishra
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-21     Completed Date:  2012-01-11     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  1025-9     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Bombay College of Pharmacy, Mumbai University, India.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents, Phytogenic / chemistry,  pharmacology*
Apoptosis / drug effects*
Benzoquinones / chemistry,  pharmacology*
Breast Neoplasms / drug therapy,  pathology*,  radiotherapy
Cell Cycle Checkpoints / drug effects*
Cell Line, Tumor
Cell Proliferation / drug effects
Female
Humans
Nigella sativa / chemistry
Radiation, Ionizing*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Benzoquinones; 490-91-5/thymoquinone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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