Document Detail


Radiosensitivity of mesothelioma cell lines.
MedLine Citation:
PMID:  8695160     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study was carried out in order to examine the radiosensitivity of malignant pleural mesothelioma cell lines. Cell kinetics, radiation-induced delay of the cell cycle and DNA ploidy of the cell lines were also determined. For comparison an HeLa and a human foetal fibroblast cell line were simultaneously explored. Six previously cytogenetically and histologically characterized mesothelioma tumor cell lines were applied. A rapid tiazolyl blue microtiter (MTT) assay was used to analyze radiosensitivity and cell kinetics and DNA ploidy of the cultured cells were determined by flow cytometry. The survival fraction after a dose of 2 Gy (SF2), parameters alpha and beta of the linear quadratic model (LQ-model) and mean inactivation dose (D(MID)) were also estimated. The DNA index of four cell lines equaled 1.0 and two cell lines equaled 1.5 and 1.6. Different mesothelioma cell lines showed a great variation in radiosensitivity. Mean survival fraction after a radiation dose of 2 Gy (SF2) was 0.60 and ranged from 0.36 to 0.81 and mean alpha value was 0.26 (range 0.48 - 0.083). The SF2 of the most sensitive diploid mesothelioma cell line was 0.36: less than that of the foetal fibroblast cell line (0.49). The survival fractions (0.81 and 0.74) of the two most resistant cell lines, which also were aneuploid, were equal to that of the HeLa cell line (0.78). The alpha/beta ratios of the most sensitive cell lines were almost an order of magnitude greater than those of the two most resistant cell lines. Radiation-induced delay of the most resistant aneuploid cell line was similar to that of HeLa cells but in the most sensitive (diploid cells) there was practically no entry into the G1 phase following the 2 Gy radiation dose during 36 h.
Authors:
A M Häkkinen; A Laasonen; K Linnainmaa; K Mattson; S Pyrhönen
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta oncologica (Stockholm, Sweden)     Volume:  35     ISSN:  0284-186X     ISO Abbreviation:  Acta Oncol     Publication Date:  1996  
Date Detail:
Created Date:  1996-09-03     Completed Date:  1996-09-03     Revised Date:  2009-05-12    
Medline Journal Info:
Nlm Unique ID:  8709065     Medline TA:  Acta Oncol     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  451-6     Citation Subset:  IM    
Affiliation:
Department of Oncology, University Central Hospital, Helsinki, Finland.
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MeSH Terms
Descriptor/Qualifier:
Aneuploidy
Cell Cycle / radiation effects
Cell Line
Cell Survival
Coloring Agents / diagnostic use
DNA, Neoplasm / radiation effects
Diploidy
Dose-Response Relationship, Radiation
Fetus
Fibroblasts / radiation effects
Flow Cytometry
G1 Phase / radiation effects
Hela Cells / radiation effects
Humans
Linear Models
Mesothelioma / radiotherapy*
Pleural Neoplasms / radiotherapy*
Ploidies
Radiation Tolerance*
Radiotherapy Dosage
Tetrazolium Salts / diagnostic use
Thiazoles / diagnostic use
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Coloring Agents; 0/DNA, Neoplasm; 0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue

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