Document Detail


The radiosensitivity of satellite cells: cell cycle regulation, apoptosis and oxidative stress.
MedLine Citation:
PMID:  20726709     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Skeletal muscles are the organ of movement, and their growth, regeneration and maintenance are dependent in large part on a population of myogenic stem cells known as satellite cells. Skeletal muscles and these resident myogenic stem cells (i.e., satellite cells) are commonly exposed to significant doses of radiation during diagnostic procedures and/or during the radiotherapeutic management of cancer. The main objective of this study was to examine the effects of clinically relevant doses of γ radiation on satellite cell survival and proliferation, cell cycle regulation, apoptosis, DNA double-strand break repair, oxidative stress and NO production. Overall, our findings demonstrate that doses of γ radiation ≥5 Gy reduced satellite cell numbers by at least 70% due in part to elevated apoptosis and the inhibition of cell cycle progression. Radiation was also found to cause a significant and persistent increase in the level of reactive oxygen and nitrogen species. Interestingly, and within this backdrop of elevated oxidative stress, similar doses were found to produce substantial reductions in the levels of nitric oxide (NO). Proliferation of satellite cells has been shown to depend in part on the production of NO, and our findings give rise to the possibility that radiation-induced reductions in NO levels may provide a mechanism for the inhibition of satellite cell proliferation in vitro and possibly the regrowth of skeletal muscle exposed during clinical irradiation procedures.
Authors:
Vincent J Caiozzo; Erich Giedzinski; Mike Baker; Tatiana Suarez; Atefeh Izadi; Mary Lan; Jennie Cho-Lim; Bertrand P Tseng; Charles L Limoli
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-08-02
Journal Detail:
Title:  Radiation research     Volume:  174     ISSN:  1938-5404     ISO Abbreviation:  Radiat. Res.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-19     Completed Date:  2010-11-12     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  582-9     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / radiation effects*
Cell Cycle / radiation effects*
Cell Proliferation / radiation effects
Cell Survival / radiation effects
DNA Breaks, Double-Stranded / radiation effects
Dose-Response Relationship, Radiation
Female
Gamma Rays
Histones / metabolism
Kinetics
Nitric Oxide / biosynthesis
Oxidative Stress / radiation effects*
Rats
Satellite Cells, Skeletal Muscle / cytology*,  metabolism,  radiation effects*
Signal Transduction / radiation effects
Grant Support
ID/Acronym/Agency:
HD50837/HD/NICHD NIH HHS; R24 HD050837/HD/NICHD NIH HHS; R24 HD050837-01/HD/NICHD NIH HHS; R24 HD050837-02/HD/NICHD NIH HHS; R24 HD050837-03/HD/NICHD NIH HHS; R24 HD050837-04/HD/NICHD NIH HHS; R24 HD050837-05/HD/NICHD NIH HHS; R24 HD050837-06/HD/NICHD NIH HHS; R24 HD050837-07/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Histones; 31C4KY9ESH/Nitric Oxide
Comments/Corrections

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