Document Detail


Radioprotective role of H(2)S/CSE pathway in Chang liver cells.
MedLine Citation:
PMID:  22982226     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Radiation-induced liver cell damage may be life-threatening. Here, we investigated whether hydrogen sulfide (H(2)S)/cystathionine γ-lyase (CSE) pathway could serve the protective role toward radiation in normal human liver cells. Our data showed that pretreatment of cells with H(2)S donor, sodium hydrosulfide (NaHS) significantly attenuated radiation induced micronuclei formation and improved cell viability. However, the use of dl-propargylglycine (PPG), a potent inhibitor of CSE, markedly enhanced the cell-killing effect induced by radiation. Exposure of cells to 2Gy γ-radiation led to significant increases of the endogenous H(2)S content. The mRNA and protein expressions of CSE also increased after radiation in a time-dependent manner, while the expression of cystathionine β-synthase (CBS), another endogenous H(2)S synthetase, did not change significantly. Notably, radiation induced production of reactive oxygen species (ROS) was significantly reversed by the pretreatment of NaHS, while blockage of CSE activity resulted in an enhanced ROS production in irradiated cells. Moreover, NaHS markedly suppressed radiation-induced phosphorylation of P53, decrease of Bcl-2/Bax, and activity of nuclear factor kappaB (NF-κB). In conclusion, our finding demonstrates that H(2)S/CSE pathway plays a radioprotection role by inhibiting radiation-induced ROS production, P53 phosphorylation, NF-κB activation and decrease of Bcl-2/Bax, indicating that modulation of H(2)S may be a novel protection strategy for liver radiation injury in radiotherapy.
Authors:
Yan Pan; Shuang Ye; Dexiao Yuan; Jianghong Zhang; Yang Bai; Chunlin Shao
Related Documents :
3511846 - Deoxyribonucleotide biosynthesis in yeast: assay and properties of ribonucleotide reduc...
663736 - Concurrent primary pneumococcemia, disseminated intravascular coagulation, and sickle c...
6817676 - Extraction of capsular material from pasteurella haemolytica.
6163216 - Induction of hemoglobin accumulation in human k562 cells by hemin is reversible.
24216166 - Single-agent obatoclax (gx15-070) potently induces apoptosis and pro-survival autophagy...
18790596 - Meso-tetra (carboxyphenyl) porphyrin (tcpp) nanoparticles were internalized by sw480 ce...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-7
Journal Detail:
Title:  Mutation research     Volume:  -     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
Institute of Radiation Medicine, Fudan University, No. 2094 Xie-Tu Road, Shanghai 200032, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Melatonin may play a role in modulation of bax and bcl-2 expression levels to protect rat peripheral...
Next Document:  Identification of amino acid residues essential to the activity of lyase CpcT1 from Nostoc sp. PCC71...