Document Detail


Radioprotective effect of N-acetylcysteine in vitro using the induction of DNA breaks as end-point.
MedLine Citation:
PMID:  7901297     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cultures of Chinese hamster cells were treated with N-acetylcysteine (NAC) and irradiated under hypoxic or aerobic conditions. Protection factors (PFs) were calculated using the yield of DNA breaks as a measure of the radiation response. With NAC up to 50 mmol dm-3, the PF values were smaller for aerobically- than for hypoxically-irradiated cells. In contrast with NAC concentrations > 100 mmol dm-3, the PF values for aerobically-irradiated cells were larger than those for hypoxic cells. This reversal is in agreement with the predictions of a modified competition model, the 'X-model'. The value of X (the ratio of reparable to non-reparable damage) was calculated to be close to 5 for the NAC-treated cells, i.e. about 18% of the radiation damage was not reparable. The increase of the total non-protein bound thiols (NPSH) and GSH content after NAC treatment was measured both in whole cells and isolated nuclei. Measurement of the cellular NPSH content showed a continuous increase with increasing NAC concentrations in the medium, independent of treatment time. In contrast, the amount of intracellular and intranuclear GSH increased with prolonged incubation time with NAC. No quantitative relationship was found between PF values and cellular NPSH or GSH content.
Authors:
G Sölen
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of radiation biology     Volume:  64     ISSN:  0955-3002     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1993-11-29     Completed Date:  1993-11-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  359-66     Citation Subset:  IM; S    
Affiliation:
Department of Tumor Biology II, Karolinska Institute, Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Acetylcysteine / pharmacology*
Animals
Cell Line
Cricetinae
Cricetulus
DNA / drug effects*,  radiation effects*
DNA Damage*
Radiation-Protective Agents / pharmacology*
Chemical
Reg. No./Substance:
0/Radiation-Protective Agents; 616-91-1/Acetylcysteine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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