| Radiolabeling of and macromolecular syntheses in Neisseria gonorrhoeae types 1 and 4. | |
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MedLine Citation:
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PMID: 403859 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Type 1 and type 4 cells of Neisseria gonorrhoeae were grown in a liquid medium that maintained colony type for several hours. Organisms from both types of colonies incorporated labeled glucose, as well as nucleic acid and protein precursors, into specific cellular fractions during growth in the medium. Ribonucleic acid and protein were synthesized actively by the bacteria, with incorporation of label chased by the addition of unlabeled precursors. The method can be used to label type 1 and type 4 cells of gonococci with specific isotopes for studies of metabolic and biochemical changes. |
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Authors:
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D V Lim; A N James; R P Williams |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 33 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 1977 Feb |
Date Detail:
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Created Date: 1977-05-20 Completed Date: 1977-05-20 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 328-33 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine
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metabolism Alanine / metabolism Autoradiography / methods* Bacterial Proteins / biosynthesis* Carbon Radioisotopes Glucose / metabolism Histidine / metabolism Neisseria gonorrhoeae / metabolism* Proline / metabolism RNA, Bacterial / biosynthesis* Tritium Uridine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Carbon Radioisotopes; 0/RNA, Bacterial; 10028-17-8/Tritium; 147-85-3/Proline; 50-99-7/Glucose; 56-41-7/Alanine; 58-96-8/Uridine; 71-00-1/Histidine; 73-24-5/Adenine |
| Comments/Corrections | |
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