| Radiolabeling of MAG3-morpholino oligomers with 188Re at high labeling efficiency and specific radioactivity for tumor pretargeting. | |
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MedLine Citation:
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PMID: 16730997 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We are investigating a novel pretargeting approach involving an initial IV injection of antitumor antibody conjugated with a phosphorodiamidate morpholino oligomer (MORF, a DNA analog) and the subsequent IV injection of the radiolabeled complement oligomer (cMORF). In this paper, the cMORF was labeled with (188)Re using MAG(3) as chelator for therapeutic applications. Since (c)MORFs are unstable in acidic condition, an optimal labeling pH was first selected and the other labeling factors were then examined. A labeling efficiency of greater than 90% can be achieved even at a concentration of MAG(3)-cMORF as low as 0.8 microM. The labeled cMORF is stable and capable of hybridizing to its complement. |
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Authors:
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Guozheng Liu; Shuping Dou; Jiang He; Dongguang Yin; Suresh Gupta; Surong Zhang; Yi Wang; Mary Rusckowski; Donald J Hnatowich |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2006-05-30 |
Journal Detail:
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Title: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine Volume: 64 ISSN: 0969-8043 ISO Abbreviation: Appl Radiat Isot Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-08-02 Completed Date: 2006-10-05 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9306253 Medline TA: Appl Radiat Isot Country: England |
Other Details:
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Languages: eng Pagination: 971-8 Citation Subset: IM |
Affiliation:
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Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA. guozheng.liu@umassmed.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, High Pressure Liquid Isotope Labeling / methods* Mice Morpholines / chemistry* Oligopeptides / isolation & purification* Organometallic Compounds / isolation & purification* Radioisotopes / isolation & purification* Radiopharmaceuticals / chemistry*, pharmacokinetics Rhenium / isolation & purification* |
| Grant Support | |
ID/Acronym/Agency:
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CA107360/CA/NCI NIH HHS; CA94994/CA/NCI NIH HHS; R21 CA107360-02/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Morpholines; 0/Oligopeptides; 0/Organometallic Compounds; 0/Radioisotopes; 0/Radiopharmaceuticals; 0/phosphorodiamidate morpholino oligomer; 0/rhenium-mercaptoacetyltriglycine; 7440-15-5/Rhenium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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