| Radioiodination of S-type lipopolysaccharide. Possibilities and limitations of labelling of the carbohydrate chain by periodate oxidation. | |
| | |
MedLine Citation:
|
PMID: 1315597 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
S-type Lipopolysaccharide (LPS) from E. coli O 139:K 82 (B) was radiolabelled by coupling 125I-tyramine to the carbohydrate chain of LPS after oxidation of sugar residues carrying vicinal hydroxy groups, with sodium metaperiodate. The specific activity amounted to 1.3 kBq/microgram LPS. As LPS is a mixture of molecules with different carbohydrate chain lengths that are associated to large micelles of high kinetic stability, the preparation and the properties of the tracer exhibit some peculiarities. Most important, partial cleavage of the carbohydrate chains of LPS is caused on radioiodination by the procedure described. As a consequence, the tracer differs markedly from the starting LPS with respect to carbohydrate chain length distribution. Another feature of special interest is the variation of the specific activity (radioactivity/unit mass) for LPS molecules of different carbohydrate chain lengths. Because of the kinetic stability of LPS micelles, the equilibration of radioiodinated and non-radioactive LPS aggregates requires their solubilization by a detergent and its subsequent removal by dialysis. It could be demonstrated that short-chain LPS molecules predominantly associate to larger micelles than long-chain molecules. Regardless of these restrictions, the 125I-LPS proved to be useful for certain analytical purposes. |
| | |
Authors:
|
U Lachmann; W Schmidt; H Wunderlich; H Schäfer |
Related Documents
:
|
16525587 - Thrombelastography for the monitoring of lipopolysaccharide induced activation of coagu... 2040687 - Lipopolysaccharide from escherichia coli reduces antigen-induced bronchoconstriction in... 10694237 - Lipopolysaccharide activates nuclear factor kappab in rat intestine: role of endogenous... 1723917 - Coronary vasodilatation induced by endotoxin in the rabbit isolated perfused heart is n... 7115637 - Aggregation of human platelets by amniotic fluid. 16525577 - Autosomal dominant thrombocytopenias with reduced expression of glycoprotein ia. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Zentralblatt für Bakteriologie : international journal of medical microbiology Volume: 276 ISSN: 0934-8840 ISO Abbreviation: Zentralbl. Bakteriol. Publication Date: 1992 Feb |
Date Detail:
|
Created Date: 1992-06-11 Completed Date: 1992-06-11 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9203851 Medline TA: Zentralbl Bakteriol Country: GERMANY |
Other Details:
|
Languages: eng Pagination: 313-22 Citation Subset: IM |
Affiliation:
|
Zentralinstitut für Isotopen- und Strahlenforschung, Berlin. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Carbohydrate Metabolism* Chromatography, Gel Electrophoresis, Polyacrylamide Gel Escherichia coli* Hydrogen-Ion Concentration Isotope Labeling / methods* Lipopolysaccharides / chemistry*, isolation & purification, metabolism Micelles Oxidation-Reduction Periodic Acid / metabolism* Sodium Dodecyl Sulfate |
| Chemical | |
Reg. No./Substance:
|
0/Lipopolysaccharides; 0/Micelles; 10450-60-9/Periodic Acid; 151-21-3/Sodium Dodecyl Sulfate; 7790-28-5/metaperiodate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Itraconazole in antifungal therapy.
Next Document: Effects of white light on the pineal gland of the chick embryo.