| Radioimmunological determination of reverse triiodothyronine in unextracted serum and serum dialysates. | |
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MedLine Citation:
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PMID: 601517 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sensitive radioimmunoassay for measurements of 3,3'-5'-triiodothyronine (reverse T3, rT3) in small amounts of unextracted serum is described. The interference of rT3 binding proteins in serum was precluded by addition of 8-anilino-1-naphthalene sulphonic acid (ANS). The cross reaction of T4 with the rT3 anti-serum varied with the concentration of T4 in the samples. At 50 per cent inhibition of [125I]rT3 binding, the T4 cross reaction was 0.075% (mol/mol). All values were corrected for T4 cross reaction. By the present method total rT3 averaged 0.37 nmol/l in thirty-two normal subjects. Higher values (0.81-1.98 nmol/l) were obtained in seventeen thyrotoxic patients, while the rT3 was very low (less than or equal to 0.046 nmol/l) in ten patients with severe primary hypothyroidism. A modification of the total rT3 assay was used for measurements of rT3 in serum dialysates (free rT3). The sensitivity was 0.46 pmol/l. This sensitivity did not allow detection of free rT3 in all normal subjects. |
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Authors:
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P Laurberg; J Weeke |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Scandinavian journal of clinical and laboratory investigation Volume: 37 ISSN: 0036-5513 ISO Abbreviation: Scand. J. Clin. Lab. Invest. Publication Date: 1977 Dec |
Date Detail:
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Created Date: 1978-03-29 Completed Date: 1978-03-29 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0404375 Medline TA: Scand J Clin Lab Invest Country: NORWAY |
Other Details:
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Languages: eng Pagination: 735-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cross Reactions Dialysis Female Humans Male Radioimmunoassay / methods* Thyroxine Triiodothyronine / blood* |
| Chemical | |
Reg. No./Substance:
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6893-02-3/Triiodothyronine; 7488-70-2/Thyroxine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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