Document Detail

Radioimmunoassay of lysergic acid diethylamide (LSD) in serum and urine by using antisera of different specificities.
MedLine Citation:
PMID:  832379     Owner:  NLM     Status:  MEDLINE    
We raised high-titre antisera to two LSD-bovine serum albumin conjugates, one linked via the indole nitrogen, the other via the amide side-chain. The antisera were specific for different parts of the LSD molecule, as demonstrated by cross-reactivity studies with LSD, its metabolites, ergot alkoloids, and closely related compounds. The antisera were used to develop a double-antibody radioimmunoassay with a detection limit of about 0.4 mug of LSD per liter of unextracted urine or serum. We saw no nonspecific interference by urine, serum, or from a series of commonly used drugs. There was good correlation between immunoassay values obtained with the two antisera (r = 0.91). However, the antiserum linked via the indole nitrogen gave consistently higher results for samples from persons who had taken LSD, owing to greater cross-reactivity with LSD metabolites. Radioimmunoassay by use of two such antisera is a more specific screening procedure for LSD abuse than has been available previously. In addition, antisera cross-reacting with LSD metabolites allow measurement of these compounds, for which there is no satisfactory method at the concentrations found in biological fluids in man.
W A Ratcliffe; S M Fletcher; A C Moffat; J G Ratcliffe; W A Harland; T E Levitt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  23     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1977 Feb 
Date Detail:
Created Date:  1977-03-31     Completed Date:  1977-03-31     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  169-74     Citation Subset:  IM    
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MeSH Terms
Antibody Specificity
Immune Sera
Lysergic Acid Diethylamide / analysis*,  blood,  urine
Protein Binding
Serum Albumin, Bovine
Reg. No./Substance:
0/Immune Sera; 0/Serum Albumin, Bovine; 50-37-3/Lysergic Acid Diethylamide

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