| Radioimmunoassay for human T-cell associated antigens on MOLT 4 cultured cells. | |
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MedLine Citation:
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PMID: 816736 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have detected antigens associated with two malignant T-cell lines by use of a sensitive radioimmunoassay. Baboon antisera to a cultured malignant T cell line (MOLT 4F) were prepared and the gammaG-anti-MOLT 4F was enriched by specific adsorption onto, and elution from, MOLT 4F cells followed by repeated absorption with pools of 21 established, long-term cultured B cell lines. Purified 125I-gammaG-anti-MOLT 4F preparation was tested for relative binding to autologous (MOLT 4F) and allogenetic (Sommer-RPMI 8402) T cells as well as B cells, including Sommer B cells (RPMI 8392). The degree of enrichment of antibody to malignant T cell associated antigens, expressed in terms of the ratios of the amount of globulin bound by T cells relative to the amount bound by B cells, were 6.3 and 5.0 for MOLT 4F and Sommer T cells, respectively. |
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Authors:
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M P Barcos; B K Seon; J Minowada; D Pressman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Immunological communications Volume: 4 ISSN: 0090-0877 ISO Abbreviation: Immunol. Commun. Publication Date: 1975 |
Date Detail:
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Created Date: 1976-07-06 Completed Date: 1976-07-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0353016 Medline TA: Immunol Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 579-86 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / isolation & purification Antigens / analysis* Binding Sites, Antibody Cells, Cultured Haplorhini Humans Papio Radioimmunoassay T-Lymphocytes / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Antigens; 0/Binding Sites, Antibody |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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