| Radioimmunoassay of epitestosterone: methodology, thermodynamic aspects and applications. | |
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MedLine Citation:
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PMID: 3695520 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A radioimmunoassay system for 17 alpha-hydroxy-4-androstene-3-one (epitestosterone) was developed and evaluated using rabbit antisera against 17 alpha-hydroxy-4-androstene-3-one-3-(O-carboxymethyl)-oxime-bovine serum albumin conjugate, and radioiodinated homologous histaminyl derivative of epitestosterone as a tracer. Two antisera with different specificity were used for radioimmunological determination of epitestosterone in hydrolyzed urine and plasma, respectively. Apparent intrinsic association constants and derived thermodynamic variables for ligand-antibody interaction were measured under various conditions in order to set up an optimal assay protocol. The mean plasma epitestosterone levels for healthy subjects were 0.44 +/- 0.15 nmol.l-1 (means +/- SD) in males and 0.092 +/- 0.056 nmol.l-1 in females. The corresponding concentrations in urine were 320 +/- 70 nmol.l-1 (males) and 273 +/- 100 nmol.l-1 (females). |
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Authors:
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R Bílek; R Hampl; Z Putz; L Stárka |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of steroid biochemistry Volume: 28 ISSN: 0022-4731 ISO Abbreviation: J. Steroid Biochem. Publication Date: 1987 Dec |
Date Detail:
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Created Date: 1988-02-20 Completed Date: 1988-02-20 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0260125 Medline TA: J Steroid Biochem Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 723-9 Citation Subset: IM |
Affiliation:
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Research Institute of Endocrinology, Praha, Czechoslovakia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Body Fluids
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analysis Epitestosterone / analysis*, immunology Female Humans Immune Sera / immunology Male Radioimmunoassay Testosterone / analysis* Thermodynamics Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Immune Sera; 481-30-1/Epitestosterone; 58-22-0/Testosterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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