Document Detail


Radioimmunoassay of the attack complex of complement in serum from patients with systemic lupus erythematosus.
MedLine Citation:
PMID:  4000197     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We developed a radioimmunoassay to measure the attack complex of complement (SC5b-9) in serum from patients with systemic lupus erythematosus. The radioimmunoassay used a monoclonal antibody to an antigen of C9, which is absent from native C9 but present on SC5b-9. SC5b-9 was detectable in 13 of 63 normal subjects, with a mean value of 0.5 unit (range, 0 to 4.8), and elevated in 13 of 14 patients with active lupus. Analysis of 108 samples from the patients with lupus revealed a mean value of 10.1 units (range, 0 to 35) when the disease was active and 1.0 unit (range, 0 to 433) when it was clinically stable. SC5b-9 was a more sensitive measure of disease activity than C3, C4, or CH50; its specificity was equivalent to that of C3 and C4. SC5b-9 was elevated in serum from 6 of 49 patients with other forms of glomerulonephritis. Our study documents the presence of SC5b-9 in abnormal serum and correlates its elevation with clinical disease activity in patients with active systemic lupus erythematosus.
Authors:
R J Falk; A P Dalmasso; Y Kim; S Lam; A Michael
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The New England journal of medicine     Volume:  312     ISSN:  0028-4793     ISO Abbreviation:  N. Engl. J. Med.     Publication Date:  1985 Jun 
Date Detail:
Created Date:  1985-07-10     Completed Date:  1985-07-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0255562     Medline TA:  N Engl J Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1594-9     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Antibodies, Monoclonal / diagnostic use
Bacterial Infections / immunology
Complement C9 / immunology
Complement Membrane Attack Complex
Complement System Proteins / analysis*
Glomerulonephritis / immunology
Humans
Lupus Erythematosus, Systemic / immunology*
Middle Aged
Radioimmunoassay
Grant Support
ID/Acronym/Agency:
AI-10704/AI/NIAID NIH HHS; AM-25518/AM/NIADDK NIH HHS; AM-26149/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Complement C9; 0/Complement Membrane Attack Complex; 9007-36-7/Complement System Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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