| Radiogenic transformation of human mammary epithelial cells in vitro. | |
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MedLine Citation:
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PMID: 11541509 Owner: NASA Status: MEDLINE |
Abstract/OtherAbstract:
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Cancer induction by space radiations is a major concern for manned space exploration. Accurate assessment of radiation risk at low doses requires basic understanding of mechanism(s) of radiation carcinogenesis. For determining the oncogenic effects of ionizing radiation in human epithelial cells, we transformed a mammary epithelial cell line (185B5), which was immortalized by benzo(a)pyrene, with energetic heavy ions and obtained several transformed clones. These transformed cells showed growth properties on Matrigel similar to human mammary tumor cells. To better understand the mechanisms of radiogenic transformation of human cells, we systematically examined the alterations in chromosomes and cancer genes. Among 16 autosomes examined for translocations, by using fluorescence in situ hybridization (FISH) technique, chromosomes 3, 12, 13, 15, 16, and 18 appeared to be normal in transformed cells. Chromosomes 1, 4, 6, 8, and 17 in transformed cells, however, showed patterns different from those in nontransformed cells. Southern blot analyses indicated no detectable alterations in myc, ras, Rb, or p53 genes. Further studies of chromosome 17 by using in situ hybridization with unique sequence p53 gene probe and a centromere probe showed no loss of p53 gene in transformed cells. Experimental results from cell fusion studies indicated that the transforming gene(s) is recessive. The role of genomic instability and tumor suppressor gene(s) in radiogenic transformation of human breast cells remains to be identified. |
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Authors:
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T C Yang; K A Georgy; A Tavakoli; L M Craise; M Durante |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Radiation oncology investigations Volume: 3 ISSN: 1065-7541 ISO Abbreviation: Radiat Oncol Investig Publication Date: 1996 |
Date Detail:
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Created Date: 1999-03-20 Completed Date: 1999-03-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9437448 Medline TA: Radiat Oncol Investig Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 412-9 Citation Subset: S |
Affiliation:
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Radiation Biophysics Laboratory, NASA Johnson Space Center, Houston, Texas, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Breast
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pathology,
radiation effects* Cell Fusion Cell Line, Transformed Cell Transformation, Neoplastic* Chromosome Aberrations Chromosomes / genetics, radiation effects Cosmic Radiation* DNA / genetics, radiation effects Epithelium / pathology, radiation effects Female Genes, Recessive / genetics Genes, Tumor Suppressor / genetics Heavy Ions Humans In Situ Hybridization, Fluorescence Karyotyping Linear Energy Transfer* Particle Accelerators |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
| Investigator | |
Investigator/Affiliation:
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T C Yang / NASA JSC |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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