Document Detail


Radioactive Smart Probe for Potential Corrected Matrix Metalloproteinase Imaging.
MedLine Citation:
PMID:  23025637     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Although various activatable optical probes have been developed to visualize the metalloproteinases (MMPs) activities in vivo, precise quantification of the enzyme activity is limited due to the inherent scattering and attenuation (limited depth penetration) properties of optical imaging. In this investigation, a novel activatable peptide probe 64Cu-BBQ650TM-PLGVR-K(Cy5.5)-E-K(DOTA)-OH was constructed to detect the tumor MMP activity in vivo. This agent is optically quenched in its native form, but releases strong fluorescence upon cleavage by selected enzymes. MMP cleavage specificity was confirmed both in vitro and in vivo by fluorescent imaging studies. The introduction of PET modality would make it feasible to correct the enzyme activity determined from optical imaging. In conclusion, the use of single modality to image biomarkers/processes may make the obtained information lack of detail and misleading in many cases. A coincident measurement technique using different imaging modality could be advantageous. In this proof of principle report, we demonstrated the feasibility of correcting the activatable optical imaging data through the PET signal. This approach stands as an attractive new strategy for accurate imaging of MMP activities, which can also be applied for other proteases imaging.
Authors:
Chiun-Wei Huang; Zibo Li; Peter S Conti
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-2
Journal Detail:
Title:  Bioconjugate chemistry     Volume:  -     ISSN:  1520-4812     ISO Abbreviation:  Bioconjug. Chem.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010319     Medline TA:  Bioconjug Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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