Document Detail


Radio fluorination and positron emission tomography (PET) as a new approach to study the in vivo distribution and elimination of the advanced glycation endproducts N epsilon-carboxymethyllysine (CML) and N epsilon-carboxyethyllysine (CEL).
MedLine Citation:
PMID:  11455785     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
After synthesis of fluorine-18 labelled analogues by [18F]fluorobenzoylation at the alpha-amino group, biodistribution and elimination of individual advanced glycation endproducts, namely N epsilon-carboxymethyllysine and N epsilon-carboxyethyllysine, were studied in comparison to lysine in rats after intravenous injection using positron emission tomography (PET). The [18F]radiofluorinated amino acids were fast distributed via the blood, followed by a rapid excretion through the kidneys. Elimination kinetics were similar for both AGEs and lysine. For CML and CEL, but not for lysine, a temporary liver accumulation could be observed, which was not connected with any metabolisation or enterohepatic circulation. No further accumulation in any tissues was observable, indicating that increased tissue levels of CML or CEL, which have been described for certain disorders, are exclusively derived from endogenous origin and should not depend on a dietary intake. However, under uremic conditions, an impaired kidney function might result in a significant increase of the AGE-load of blood and tissues. PET based on 18F-labelled AGEs proved to be a promising tool to elucidate the physiological fate of post-translationally modified amino acids and to clarify the role of AGEs as possible "glycotoxins".
Authors:
R Bergmann; R Helling; C Heichert; M Scheunemann; P Mäding; H Wittrisch; B Johannsen; T Henle
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Die Nahrung     Volume:  45     ISSN:  0027-769X     ISO Abbreviation:  Nahrung     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-07-17     Completed Date:  2001-08-09     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0142530     Medline TA:  Nahrung     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  182-8     Citation Subset:  IM    
Affiliation:
Research Centre Rossendorf, Institute of Bioinorganic and Radiopharmaceutical Chemistry, Rossendorf.
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MeSH Terms
Descriptor/Qualifier:
Animals
Fluorine Radioisotopes / diagnostic use*
Glycosylation End Products, Advanced / pharmacokinetics*,  physiology
Half-Life
Kidney / metabolism*
Liver / metabolism*
Male
Rats
Rats, Wistar
Tissue Distribution
Tomography, Emission-Computed / methods*
Chemical
Reg. No./Substance:
0/Fluorine Radioisotopes; 0/Glycosylation End Products, Advanced

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