| Radiation and thermal characteristics of mouse lymphoma cells and their radiation-sensitive mutant. | |
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MedLine Citation:
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PMID: 3231725 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Radiation and thermal characteristics of L5178Y cells and their radiation-sensitive mutant M10 cells were studied by the colony-forming method and the dye-exclusion method using eosin-Y. Although M10 cells were remarkably radiation-sensitive compared with L5178Y cells, it was difficult to cause interphase death of M10 after a large dose of irradiation. After heat treatments, L5178Y cells revealed more cell destruction and were stained well by eosin-Y, but it was relatively difficult to produce cell destruction of M10 cells, which showed poor staining by eosin-Y. When assayed by the colony-forming method, M10 cells were also heat-resistant compared to L5178Y. The dye-exclusion rate was closely correlated with cell survival after hyperthermia of L5178Y cells, suggesting that this is a simple method of detecting the thermosensitivity and thermotolerance of cancer cells. The difference in survival of L5178Y cells and M10 cells after combined treatment with gamma irradiation and hyperthermia was smaller than with gamma irradiation alone. It was also found that there was a relationship between radiation-induced interphase death and hyperthermia-induced interphase death, and that interphase death accounted for a major part of cell death caused by hyperthermia in mouse leukemia cells. |
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Authors:
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Y Baba; T Yasunaga; H Uozumi; M Takahashi; S Sawada |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Radiation medicine Volume: 6 ISSN: 0288-2043 ISO Abbreviation: Radiat Med Publication Date: 1988 Sep-Oct |
Date Detail:
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Created Date: 1989-04-20 Completed Date: 1989-04-20 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8412264 Medline TA: Radiat Med Country: JAPAN |
Other Details:
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Languages: eng Pagination: 232-9 Citation Subset: IM |
Affiliation:
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Department of Radiology, Kumamoto University Medical School, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Survival Cobalt Radioisotopes Hot Temperature* Leukemia L5178 / pathology* Leukemia, Experimental / pathology* Mice Mutation* Neoplastic Stem Cells / cytology, radiation effects Radiation Tolerance Tumor Cells, Cultured* / cytology, radiation effects |
| Chemical | |
Reg. No./Substance:
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0/Cobalt Radioisotopes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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