| Radiation resistance and virulence of Listeria monocytogenes Scott A following starvation in physiological saline. | |
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MedLine Citation:
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PMID: 15035359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The influence of starvation on the resistance of Listeria monocytogenes Scott A to electron beam irradiation in 0.85% (wt/vol) NaCl (saline) and in ground pork was investigated. Exponential- or stationary-phase cells (control) were grown at 35 degrees C in tryptic soy broth supplemented with 0.6% yeast extract. Washed cells were starved for 12 days in saline, and virulence of the pathogen was evaluated at 0, 8, and 12 days during starvation. Samples of saline and irradiation-sterilized ground pork, inoculated with control or starved cells, were irradiated at doses ranging from 0.0 to 2.5 kGy. L. monocytogenes survivors were determined by plating diluted samples of saline or pork on tryptic soy agar supplemented with 0.6% yeast extract and counting bacterial colonies following incubation (35 degrees C, 48 h). Virulence of starved cells and control was not significantly different (P > 0.05). Cells exhibited the highest radiation resistance at 8 days of starvation. Irradiation (0.5 kGy) in saline resulted in approximately 7.14, 5.55, and 2.38 log reduction in exponential, stationary, and starved cells, respectively. Irradiation of ground pork at 2.5 kGy reduced controls by approximately 6.0 log, whereas starved cells were reduced by only 3.8 log. Starved cells consistently exhibited higher irradiation D10-values than controls (P < 0.05). D10-values for exponential, stationary, and starved cells were 0.07, 0.09, and 0.21 kGy and 0.35, 0.42, and 0.66 kGy in saline and ground pork, respectively. These results indicate that starvation cross-protects L. monocytogenes Scott A against radiation inactivation and should be considered when determining this pathogen's irradiation D-value. |
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Authors:
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A F Mendonca; M G Romero; M A Lihono; R Nannapaneni; M G Johnson |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of food protection Volume: 67 ISSN: 0362-028X ISO Abbreviation: J. Food Prot. Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-03-23 Completed Date: 2004-04-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7703944 Medline TA: J Food Prot Country: United States |
Other Details:
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Languages: eng Pagination: 470-4 Citation Subset: IM |
Affiliation:
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Iowa State University, Department of Food Science and Human Nutrition, 2312 Food Sciences Building, Ames, Iowa 50011, USA. amendon@iastate.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological Animals Colony Count, Microbial Consumer Product Safety Culture Media / chemistry Dose-Response Relationship, Radiation Food Irradiation* Food Microbiology Gamma Rays Listeria monocytogenes / growth & development, pathogenicity*, radiation effects* Meat Products / microbiology* Swine Time Factors Virulence / physiology |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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