Document Detail


Radiation-reduced hybrids for the myotonic dystrophy locus.
MedLine Citation:
PMID:  1612584     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The myotonic dystrophy (DM) gene maps to the long arm of human chromosome 19 and is flanked by markers ERCC1 and D19S51. Also mapping to this region is the polio virus receptor gene (PVS). To produce more markers for this interval, we have constructed radiation-reduced hybrids by selecting for the retention of ERCC1 and for the loss of PVS. One of the cell lines produced has been characterized extensively and contains about 2 Mb of human DNA derived exclusively from chromosome 19, and includes ERCC1 and D19S51. Phage libraries constructed from DNA of this cell line have been screened and several new markers identified, including two for which cDNAs have been isolated. These represent candidate genes for DM. The new markers have also been used to extend the long-range restriction map of this region.
Authors:
J D Brook; B V Zemelman; K Hadingham; M J Siciliano; S Crow; H G Harley; S A Rundle; J Buxton; K Johnson; J W Almond
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genomics     Volume:  13     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-07-30     Completed Date:  1992-07-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  243-50     Citation Subset:  IM    
Affiliation:
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02138.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Cell Line / radiation effects
Chromosome Mapping / methods*
Chromosomes, Human, Pair 19*
DNA / genetics
Gene Library
Genes, Dominant*
Genetic Markers*
Humans
Hybrid Cells
Incidence
Molecular Sequence Data
Myotonic Dystrophy / epidemiology,  genetics*
Restriction Mapping
Selection, Genetic
Grant Support
ID/Acronym/Agency:
HG-00299/HG/NHGRI NIH HHS; HL-41484/HL/NHLBI NIH HHS; //Wellcome Trust
Chemical
Reg. No./Substance:
0/Genetic Markers; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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