| Radiation-induced premature senescence is associated with specific cytogenetic changes. | |
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MedLine Citation:
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PMID: 20338260 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present study, we set out to investigate cytogenetic changes in the progeny of two normal human fibroblast cell strains after exposure to sparsely or densely ionizing irradiation (X-rays or 9.8 MeV u(-1) carbon ions). The cells were regularly subcultured up to senescence. The transition to senescence was determined by measurement of population doubling numbers and senescence associated (SA) beta-galactosidase activity. Chromosomal changes (structural aberrations, tetraploidy) were investigated by solid staining. In temporal proximity to senescence, we observed for all populations of the two fibroblasts cell strains an increase in the fraction of cells with structural and numerical aberrations. The observed changes in the yield of structural chromosomal aberrations were similar for the progeny of controls and irradiated cells, except that a previous irradiation with a high, fractionated X-ray dose resulted in a stronger increase. Noteworthy, delayed tetraploidy in the descendants of irradiated cells exceeded the level in control cells. In addition, tetraploidy and the time of onset of senescence were significantly correlated for all populations, regardless of a preceding radiation exposure. However, the time of the onset of senescence depends on previous exposure to radiation. We conclude that the occurrence of tetraploidy is associated with senescence independently of exposure to radiation. |
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Authors:
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Sebastian Zahnreich; Larisa Melnikova; Marcus Winter; Elena Nasonova; Marco Durante; Sylvia Ritter; Claudia Fournier |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-23 |
Journal Detail:
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Title: Mutation research Volume: 701 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-26 Completed Date: 2010-10-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 60-6 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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GSI, Biophysics, Darmstadt, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbon Cell Aging / radiation effects* Cell Proliferation Cells, Cultured Chromosome Aberrations* Fibroblasts / radiation effects Foreskin / cytology Genomic Instability Heavy Ions Humans Male Polyploidy beta-Galactosidase / analysis |
| Chemical | |
Reg. No./Substance:
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7440-44-0/Carbon; EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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