Document Detail

Radiation-induced association of beta-glucuronidase with purified nuclei from irradiated MOLT-4 and HeLa cells.
MedLine Citation:
PMID:  2922468     Owner:  NLM     Status:  MEDLINE    
Beta-glucuronidase, a lysosomal marker enzyme, associates with purified nuclei from HeLa and MOLT-4 cell lines in a radiation dose-dependent manner, up to 300 cGy in MOLT-4 cells, and 1000 cGy in HeLa cells. In MOLT-4 cells (200-cGy exposure), there is a significant increase in beta-glucuronidase activity detected in the nuclear fraction 24 h postirradiation with a maximum association occurring at 72 h. In HeLa cells (1000-cGy exposure), a significant association is first detected 24 h postirradiation with a maximum association at 48 h. The association is not the result of nonspecific contamination occurring during nuclei purification since nuclei from irradiated cells show no greater levels of plasma membrane marker and mitochondrial marker than controls. The nature of the association remains unclear, but activity is not removed by detergents used in the nuclei isolation procedure, and incubation of the nuclei with EDTA reverses the association only modestly. Exposure of nuclei from irradiated cells to anisotonic buffers also results in only a small decrease in beta-glucuronidase activity associated with the nuclei. These observations suggest that lysosomal hydrolases become intimately associated with the nuclei of irradiated cells.
D E McClain; J F Kalinich; J K Poplack; S L Snyder
Related Documents :
16494518 - Mechanism of porphyrin-induced sonodynamic effect: possible role of hyperthermia.
1225718 - Potentiation of cytostatic effect of sulfur- or chlorine-containing purines and related...
15364548 - Proteasome-dependent degradation of cytochromes p450 2e1 and 2b1 expressed in tetracycl...
10358078 - Dexamethasone alters arachidonate release from human epithelial cells by induction of p...
8887268 - Role of cytochrome p-450 in hydrogen peroxide-induced cytotoxicity to llc-pk1 cells.
17987128 - Gene expression analysis of in vivo fluorescent cells.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Radiation research     Volume:  117     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-04-17     Completed Date:  1989-04-17     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  221-33     Citation Subset:  IM; S    
Radiation Biochemistry Department, Armed Forces Radiobiology Research Institute, Bethesda, Maryland 20814-5145.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Line
Cell Nucleus / enzymology,  radiation effects*
Cell Survival / radiation effects
Cobalt Radioisotopes
Glucuronidase / metabolism*
Hela Cells
Lysosomes / enzymology*
Tumor Cells, Cultured / radiation effects
Reg. No./Substance:
0/Cobalt Radioisotopes; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Analysis of structural and numerical chromosomal aberrations at the first and second mitosis after X...
Next Document:  Chromatin decondensed by acetylation shows an elevated radiation response.