Document Detail


Radiation-induced association of beta-glucuronidase with purified nuclei from irradiated MOLT-4 and HeLa cells.
MedLine Citation:
PMID:  2922468     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Beta-glucuronidase, a lysosomal marker enzyme, associates with purified nuclei from HeLa and MOLT-4 cell lines in a radiation dose-dependent manner, up to 300 cGy in MOLT-4 cells, and 1000 cGy in HeLa cells. In MOLT-4 cells (200-cGy exposure), there is a significant increase in beta-glucuronidase activity detected in the nuclear fraction 24 h postirradiation with a maximum association occurring at 72 h. In HeLa cells (1000-cGy exposure), a significant association is first detected 24 h postirradiation with a maximum association at 48 h. The association is not the result of nonspecific contamination occurring during nuclei purification since nuclei from irradiated cells show no greater levels of plasma membrane marker and mitochondrial marker than controls. The nature of the association remains unclear, but activity is not removed by detergents used in the nuclei isolation procedure, and incubation of the nuclei with EDTA reverses the association only modestly. Exposure of nuclei from irradiated cells to anisotonic buffers also results in only a small decrease in beta-glucuronidase activity associated with the nuclei. These observations suggest that lysosomal hydrolases become intimately associated with the nuclei of irradiated cells.
Authors:
D E McClain; J F Kalinich; J K Poplack; S L Snyder
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Radiation research     Volume:  117     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-04-17     Completed Date:  1989-04-17     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  221-33     Citation Subset:  IM; S    
Affiliation:
Radiation Biochemistry Department, Armed Forces Radiobiology Research Institute, Bethesda, Maryland 20814-5145.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Cell Nucleus / enzymology,  radiation effects*
Cell Survival / radiation effects
Cobalt Radioisotopes
Glucuronidase / metabolism*
Hela Cells
Humans
Lysosomes / enzymology*
Tumor Cells, Cultured / radiation effects
Chemical
Reg. No./Substance:
0/Cobalt Radioisotopes; EC 3.2.1.31/Glucuronidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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