Document Detail

Radiation-induced apoptosis in developing rats and kainic acid-induced excitotoxicity in adult rats are associated with distinctive morphological and biochemical c-Jun/AP-1 (N) expression.
MedLine Citation:
PMID:  9284348     Owner:  NLM     Status:  MEDLINE    
Ionizing radiation produces apoptosis in the developing rat brain. Strong c-Jun immunoreactivity, as revealed with the antibody c-Jun/AP-1 (N) which is raised against the amino acids 91-105 mapping with the amino terminal domain of mouse c-Jun p39, is simultaneously observed in the nucleus and cytoplasm of apoptotic cells. Western blotting of total brain homogenates, using the same antibody, shows a p39 band in control rats which is accompanied by a strong, phosphorylated p62 double-band in irradiated animals. In addition, increased c-Jun N-terminal kinase 1 expression, as found on western blots, is found in irradiated rats when compared with controls. Intraperitoneal injection of kainic acid at convulsant doses to the adult rat produces cell death with morphological features of necrosis, together with the appearance of cells with fine granular chromatin degeneration and small numbers of apoptotic-like cells, in the entorhinal and piriform cortices, basal amygdala, certain thalamic nuclei, and CA1 region of the hippocampus. c-Jun expression in kainic acid-treated rats, as revealed with the c-Jun/AP-1 (N) antibody, is found in the nuclei of a minority of cells in the same areas. The vast majority of c-Jun-immunoreactive cells have normal nuclear morphology, whereas necrotic cells are negative and only a few cells with fine granular chromatin condensation and apoptotic cells following kainic acid injection are stained with c-Jun antibodies. Western blotting, using the same antibody, shows a p39 band in control rats, which is accompanied by a band at about p26 from 6 h onwards following kainic acid injection. Decreased c-Jun N-terminal kinase 1 expression, as revealed on western blots, is observed in kainic acid-treated rats. These results show that the antibody c-Jun/AP-1 (N) recognizes three different forms of c-Jun-related immunoreactivity in normal and pathological states, which are associated with the different outcome of cells. These results stress the necessity of examining in detail the composition of c-Jun-immunoreactive bands and the metabolic state of c-Jun(s) in different paradigms of cell death and survival.
I Ferrer; A M Planas; E Pozas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience     Volume:  80     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1997-10-17     Completed Date:  1997-10-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  449-58     Citation Subset:  IM    
Unitat de Neuropatologia, Servei d'Anatomia Patològica, Hospital Princeps d'Espanya, Universitat de Barcelona, Spain.
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MeSH Terms
Apoptosis / drug effects*,  radiation effects*
Blotting, Western
Brain / cytology,  drug effects,  radiation effects
DNA Fragmentation
Electrophoresis, Polyacrylamide Gel
Excitatory Amino Acid Agonists / toxicity*
Gene Expression Regulation / drug effects*,  radiation effects*
Kainic Acid / toxicity*
Proto-Oncogene Proteins c-jun / biosynthesis*,  drug effects,  radiation effects
Rats, Sprague-Dawley
Transcription Factor AP-1 / biosynthesis*,  drug effects,  radiation effects
Reg. No./Substance:
0/Excitatory Amino Acid Agonists; 0/Proto-Oncogene Proteins c-jun; 0/Transcription Factor AP-1; 487-79-6/Kainic Acid
Comment In:
Neuroscience. 2000;96(2):445-6   [PMID:  10683585 ]

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