| Radiation induced G1-block and p53 status in six human cell lines. | |
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MedLine Citation:
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PMID: 8539453 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Considerable attention has recently been focused on the fact that the tumor suppressor protein p53 is involved in the cellular response to radiation. In its wild-type form the protein appears to control a cell cycle checkpoint, preventing entry into S-phase following DNA damage. A number of authors observed a radiation induced G1-block in cells expressing wild-type p53, but not in p53 mutant cells. We obtained similar results with four human tumour cell lines as well as two strains of human fibroblasts, whose p53 status was ascertained at the protein as well as DNA levels. In addition to cell cycle delays in exponentially growing cell cultures, we have studied the possible role of the p53 in the transition from quiescence to active proliferation. Cells were irradiated after 6 days of serum-starvation and labelled with BrdU at different times after addition of fresh medium. Entry into S-phase was found to be delayed by several hours in the p53 wild-type cells, but no such effect was observed in the p53 mutants. Where a delay occurred, it was roughly proportional to the X-ray dose. Although it remains to be clarified, whether the cells were delayed only in G1 or also in G0, it is interesting to note that entry into S-phase can be delayed by irradiation in a quiescent state immediately before serum-stimulation, provided the cells are wild-type with respect to p53. Certain differences in the cell cycle response of transformed and untransformed cells were noted. |
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Authors:
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F Zölzer; S Hillebrandt; C Streffer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology Volume: 37 ISSN: 0167-8140 ISO Abbreviation: Radiother Oncol Publication Date: 1995 Oct |
Date Detail:
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Created Date: 1996-02-08 Completed Date: 1996-02-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8407192 Medline TA: Radiother Oncol Country: IRELAND |
Other Details:
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Languages: eng Pagination: 20-8 Citation Subset: IM |
Affiliation:
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Institut für Medizinische Strahlenbiologie, Universitätsklinikum Essen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antimetabolites
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diagnostic use Bromodeoxyuridine / diagnostic use Carcinoma, Squamous Cell / genetics, pathology, radiotherapy* Cell Cycle / radiation effects Cell Cycle Proteins / genetics, radiation effects Cell Division / genetics, radiation effects Cell Line, Transformed Culture Media, Serum-Free DNA Damage DNA, Neoplasm / genetics, radiation effects Fibroblasts / metabolism, radiation effects G0 Phase / radiation effects G1 Phase / radiation effects* Humans Male Melanoma / genetics, pathology, radiotherapy* Middle Aged Mutation / genetics Radiotherapy Dosage S Phase / radiation effects Tumor Cells, Cultured Tumor Suppressor Protein p53 / genetics, radiation effects* |
| Chemical | |
Reg. No./Substance:
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0/Antimetabolites; 0/Cell Cycle Proteins; 0/Culture Media, Serum-Free; 0/DNA, Neoplasm; 0/Tumor Suppressor Protein p53; 59-14-3/Bromodeoxyuridine |
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