Document Detail

Radiation cell survival and growth delay studies in multicellular spheroids of small-cell lung carcinoma.
MedLine Citation:
PMID:  3030949     Owner:  NLM     Status:  MEDLINE    
The radiation sensitivity of two small-cell lung carcinoma cell lines growing as multicellular spheroids in static culture was determined using clonogenic cell survival and growth delay as endpoints. Growth delay determination suggested that clonogenic cell kill was less than was obtained by direct assay of cell survival. Recovery from potentially lethal damage was assayed in one line (HC12) but was not demonstrable, and clonogenic cell survival decreased with time in treated spheroids with diameters greater than 300 microns which contained a hypoxic cell population. Microscopic examination of the treated spheroids showed the emergence of an abnormal giant-cell population, and the progressive clonogenic cell loss that occurred after treatment was thought to be due to oxygen and nutrient deprivation of the remaining viable cells by this doomed cell population. Correction of the growth delay measurements for changes in cell size and clonogenic cell population allowed correlation of the growth delay and cell survival data.
G M Duchesne; J H Peacock
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of radiation biology and related studies in physics, chemistry, and medicine     Volume:  51     ISSN:  0020-7616     ISO Abbreviation:  Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med.     Publication Date:  1987 Feb 
Date Detail:
Created Date:  1987-04-28     Completed Date:  1987-04-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0374725     Medline TA:  Int J Radiat Biol Relat Stud Phys Chem Med     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  365-75     Citation Subset:  IM    
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MeSH Terms
Carcinoma, Small Cell / pathology*,  radiotherapy
Cell Division / radiation effects
Cell Line
Cell Survival / radiation effects
Culture Techniques
Dose-Response Relationship, Radiation
Gamma Rays
Lung Neoplasms / pathology*,  radiotherapy
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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