Document Detail

Radial sizing of lipid nanotubes using membrane displacement analysis.
MedLine Citation:
PMID:  22313341     Owner:  NLM     Status:  MEDLINE    
We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracted from SPE vesicles, enabling quantification of membrane composition and lamellarity. Variances of nanotube radii were measured, showing a growth of 40-56 nm, upon increasing cholesterol content from 0 to 20%.
Natalia Stepanyants; Gavin D M Jeffries; Owe Orwar; Aldo Jesorka
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-24
Journal Detail:
Title:  Nano letters     Volume:  12     ISSN:  1530-6992     ISO Abbreviation:  Nano Lett.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-14     Completed Date:  2012-07-03     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101088070     Medline TA:  Nano Lett     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1372-8     Citation Subset:  IM    
Copyright Information:
© 2012 American Chemical Society
Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-41296 Göteborg, Sweden.
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MeSH Terms
Fluorescence Recovery After Photobleaching / methods*
Lipids / chemistry*
Macromolecular Substances / chemistry
Materials Testing
Membranes, Artificial*
Molecular Conformation
Nanotubes / chemistry*,  ultrastructure*
Particle Size
Surface Properties
Reg. No./Substance:
0/Lipids; 0/Macromolecular Substances; 0/Membranes, Artificial

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