| Radial flow bioreactor for the creation of bioartificial liver and kidney. | |
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MedLine Citation:
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PMID: 15808597 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A radial flow bioreactor (RFB) is used for a three-dimensional perfusion culture of hepatocellular carcinoma (HCC) cells and renal cells, to create a bioartificial liver and kidney. The cylindrical reactor is filled with porous cellulose microcarrier. RFB can be characterized as a system in which the medium flows from the periphery toward the center of the reactor, thereby delivering an adequate supply of oxygen and nutrients to cells at the center as well as at the periphery. HCC cells incubated in the RFB system at high density maintained viability for long periods of time. Proximal tubular cells (LLC-PK1) as well as HCC cells, but not human immortalized mesangial cells (HMC) were cultured in the RFB for more than 14 days. The mRNA expression of some enzymes involved in the urea cycle, cytochrome P450s in HCC cells, and the 1-alpha-hydroxylase (CYP27B1) in LLC-PK1 cells was higher than that in monolayer cultures. These results suggest that the RFB system composed of HCC cells or renal cells may be useful for a bioartificial liver and kidney. |
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Authors:
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T Iwahori; N Matsuno; Y Johjima; O Konno; I Akashi; Y Nakamura; K Hama; H Iwamoto; M Uchiyama; T Ashizawa; T Nagao |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Transplantation proceedings Volume: 37 ISSN: 0041-1345 ISO Abbreviation: Transplant. Proc. Publication Date: 2005 Jan-Feb |
Date Detail:
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Created Date: 2005-04-05 Completed Date: 2005-10-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0243532 Medline TA: Transplant Proc Country: United States |
Other Details:
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Languages: eng Pagination: 212-4 Citation Subset: IM |
Affiliation:
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Division of Artificial Organ and Blood Purification, 5th Department of Surgery, Hachioji Medical Center of Tokyo Medical University, Tokyo, Japan. tiwa@tokyo-med.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carcinoma, Hepatocellular / pathology* Cell Culture Techniques Cell Line Equipment Design Humans Kidney / cytology* Kidney, Artificial* Liver Neoplasms / pathology* Liver, Artificial* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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