Document Detail


Rad52 sumoylation and its involvement in the efficient induction of homologous recombination.
MedLine Citation:
PMID:  18396468     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The protein Rad52 is a key player in various types of homologous recombination and is essential to maintenance of genomic integrity. Although evidence indicates that Rad52 is modified by SUMO, the physiological relevance of this sumoylation remains unclear. Here, we identify the conditions under which Rad52 sumoylation is induced, and clarify the role of this modification in homologous recombination. Oligomerization of Rad52 was a prerequisite for sumoylation, and the modification occurred in the cell proceeding S phase being exposed to the DNA-damaging agent methyl methanesulfonate (MMS). Following exposure to MMS, sumoylated Rad52 accumulated in rad51 cells, but not in the recombination-related gene mutants, rad54, rad55, rad59, sgs1, or srs2. The accumulation of sumoylated Rad52 was suppressed in rad51 cells expressing Rad51-K191R, an ATPase-defective protein presumed to be recruited to ssDNA. Although the sumoylation defective mutant rad52-3KR (K10R/K11R/K220R) showed no defect in mating-type switching, which did not lead to Rad52 sumoylation in wild-type cells, the mutant did demonstrate a partial defect in MMS-induced interchromosomal homologous recombination.
Authors:
Takashi Ohuchi; Masayuki Seki; Dana Branzei; Daisuke Maeda; Ayako Ui; Hideaki Ogiwara; Shusuke Tada; Takemi Enomoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-08
Journal Detail:
Title:  DNA repair     Volume:  7     ISSN:  1568-7864     ISO Abbreviation:  DNA Repair (Amst.)     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-26     Completed Date:  2008-08-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139138     Medline TA:  DNA Repair (Amst)     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  879-89     Citation Subset:  IM    
Affiliation:
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6-3, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
DNA / drug effects
DNA Primers
Methyl Methanesulfonate / pharmacology
Mutagenesis, Site-Directed
Rad52 DNA Repair and Recombination Protein / genetics,  metabolism*
Recombination, Genetic*
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Small Ubiquitin-Related Modifier Proteins / metabolism*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/RAD52 protein, S cerevisiae; 0/Rad52 DNA Repair and Recombination Protein; 0/Saccharomyces cerevisiae Proteins; 0/Small Ubiquitin-Related Modifier Proteins; 66-27-3/Methyl Methanesulfonate; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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