Document Detail

A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  8769646     Owner:  NLM     Status:  MEDLINE    
With the use of an intrachromosomal inverted-repeat as a recombination reporter we have previously shown that mitotic recombination is dependent on the RAD52 gene. However, recombination was found to be reduced only 4-fold by mutation of RAD51, which encodes a homolog of bacterial RecA proteins. A rad51, which strain containing the recombination reporter was mutagenized to identify components of the RAD51-independent pathway. One mutation identified, rad59, reduced recombination 1200-fold in the presence of a rad51 mutation, but only 4- to 5-fold in a wild-type background. Thus the rad51 and rad59 mutations reduce recombination synergistically. The rad59 mutation reduced both spontaneous and double-strand-break-induced recombination between inverted repeats. However, the rate of interchromosomal recombination was increased in a rad59 homozygous diploid. These observations suggest that RAD59 functions specifically in intrachromosomal recombination. The rad59 mutant strain was sensitive to ionizing radiation, and this phenotype was used to clone the RAD59 gene by complementation. The gene encodes a protein of 238 amino acids with significant homology to members of the Rad52 family. Overexpression of RAD52 was found to suppress the DNA repair and recombination defects conferred by the rad59 mutation, suggesting that these proteins have overlapping roles or function as a complex.
Y Bai; L S Symington
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genes & development     Volume:  10     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-10-03     Completed Date:  1996-10-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2025-37     Citation Subset:  IM    
Columbia University College of Physicians and Surgeons, Department of Microbiology and Institute of Cancer Research, New York, New York 10032, USA.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA Primers / chemistry
DNA Repair*
DNA-Binding Proteins / genetics,  physiology*
Fungal Proteins / genetics*,  physiology*
Gamma Rays
Gene Conversion
Gene Expression Regulation, Fungal
Genes, Fungal
Genetic Complementation Test
Molecular Sequence Data
Rad51 Recombinase
Rad52 DNA Repair and Recombination Protein
Recombination, Genetic*
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae Proteins
Sequence Alignment
Sequence Homology, Amino Acid
Grant Support
Reg. No./Substance:
0/DNA Primers; 0/DNA-Binding Proteins; 0/Fungal Proteins; 0/RAD52 protein, S cerevisiae; 0/Rad52 DNA Repair and Recombination Protein; 0/Saccharomyces cerevisiae Proteins; EC 2.7.7.-/RAD51 protein, S cerevisiae; EC 2.7.7.-/Rad51 Recombinase

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