Document Detail

Rad52 depletion in Candida albicans triggers both the DNA-damage checkpoint and filamentation accompanied by but independent of expression of hypha-specific genes.
MedLine Citation:
PMID:  16468988     Owner:  NLM     Status:  MEDLINE    
We have analysed the effect of RAD52 deletion in several aspects of the cell biology of Candida albicans. Cultures of rad52Delta strains exhibited slow growth and contained abundant cells with a filamentous morphology. Filamentation with polarization of actin patches was accompanied by the induction of the hypha-specific genes (HSG) ECE1, HWP1 and HGC1. However, filament formation occurred in the absence of the transcription factors Efg1 and Cph1, even though disruption of EFG1 prevented expression of HSG. Therefore, expression of HSG genes accompanies but is dispensable for rad52Delta filamentation. However, deletion of adenylate cyclase severely impaired filamentation, this effect being largely reverted by the addition of exogenous cAMP. Filaments resembled elongated pseudohyphae, but some of them looked like true hyphae. Following depletion of Rad52, many cells arrested at the G2/M phase of the cell cycle with a single nucleus suggesting the early induction of the DNA-damage checkpoint. Filaments formed later, preferentially from G2/M cells. The filamentation process was accompanied by the uncoupling of several landmark events of the cell cycle and was partially dependent on the action of the cell cycle modulator Swe1. Hyphae were still induced by serum, but a large number of rad52 cells myceliated in G2/M.
Encarnación Andaluz; Toni Ciudad; Jonathan Gómez-Raja; Richard Calderone; Germán Larriba
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  59     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-10     Completed Date:  2006-05-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1452-72     Citation Subset:  IM    
Departamento de Microbiología, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain.
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MeSH Terms
Adenylate Cyclase / genetics,  metabolism
Candida albicans / genetics*,  pathogenicity,  physiology
Cell Cycle / genetics
Cell Cycle Proteins / genetics,  metabolism
Cell Nucleus / genetics
Cell Proliferation
Cyclins / genetics,  metabolism
DNA Damage / genetics*
DNA Replication / genetics
DNA-Binding Proteins / genetics,  metabolism
Fungal Proteins / genetics*,  metabolism
Gene Expression Regulation, Fungal*
Membrane Glycoproteins / genetics,  metabolism
Microfilaments / genetics,  metabolism
Protein-Tyrosine Kinases / genetics,  metabolism
Rad52 DNA Repair and Recombination Protein / genetics*,  metabolism
Transcription Factors / genetics,  metabolism
Grant Support
Reg. No./Substance:
0/CPH1 protein, Candida albicans; 0/Cell Cycle Proteins; 0/Cyclins; 0/DNA-Binding Proteins; 0/EFG1 protein, Candida albicans; 0/Fungal Proteins; 0/HWP1 protein, Candida albicans; 0/Membrane Glycoproteins; 0/Rad52 DNA Repair and Recombination Protein; 0/Transcription Factors; EC Kinases; EC Cyclase

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