| Rad52 depletion in Candida albicans triggers both the DNA-damage checkpoint and filamentation accompanied by but independent of expression of hypha-specific genes. | |
| | |
MedLine Citation:
|
PMID: 16468988 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have analysed the effect of RAD52 deletion in several aspects of the cell biology of Candida albicans. Cultures of rad52Delta strains exhibited slow growth and contained abundant cells with a filamentous morphology. Filamentation with polarization of actin patches was accompanied by the induction of the hypha-specific genes (HSG) ECE1, HWP1 and HGC1. However, filament formation occurred in the absence of the transcription factors Efg1 and Cph1, even though disruption of EFG1 prevented expression of HSG. Therefore, expression of HSG genes accompanies but is dispensable for rad52Delta filamentation. However, deletion of adenylate cyclase severely impaired filamentation, this effect being largely reverted by the addition of exogenous cAMP. Filaments resembled elongated pseudohyphae, but some of them looked like true hyphae. Following depletion of Rad52, many cells arrested at the G2/M phase of the cell cycle with a single nucleus suggesting the early induction of the DNA-damage checkpoint. Filaments formed later, preferentially from G2/M cells. The filamentation process was accompanied by the uncoupling of several landmark events of the cell cycle and was partially dependent on the action of the cell cycle modulator Swe1. Hyphae were still induced by serum, but a large number of rad52 cells myceliated in G2/M. |
| | |
Authors:
|
Encarnación Andaluz; Toni Ciudad; Jonathan Gómez-Raja; Richard Calderone; Germán Larriba |
Related Documents
:
|
10718628 - Effect of regulated expression of the fragile histidine triad gene on cell cycle and pr... 8898858 - Targeted neutralization of calmodulin in the nucleus blocks dna synthesis and cell cycl... 7730408 - A cell cycle checkpoint monitors cell morphogenesis in budding yeast. 14593748 - Cytometry of cell cycle regulatory proteins. 872138 - Treatment of multiple myeloma in remission with anticancer drugs having cell cycle spec... 20056748 - The microrna-processing enzyme dicer maintains juxtaglomerular cells. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Molecular microbiology Volume: 59 ISSN: 0950-382X ISO Abbreviation: Mol. Microbiol. Publication Date: 2006 Mar |
Date Detail:
|
Created Date: 2006-02-10 Completed Date: 2006-05-02 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: England |
Other Details:
|
Languages: eng Pagination: 1452-72 Citation Subset: IM |
Affiliation:
|
Departamento de Microbiología, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenylate Cyclase
/
genetics,
metabolism Candida albicans / genetics*, pathogenicity, physiology Cell Cycle / genetics Cell Cycle Proteins / genetics, metabolism Cell Nucleus / genetics Cell Proliferation Cyclins / genetics, metabolism DNA Damage / genetics* DNA Replication / genetics DNA-Binding Proteins / genetics, metabolism Fungal Proteins / genetics*, metabolism Gene Expression Regulation, Fungal* Membrane Glycoproteins / genetics, metabolism Microfilaments / genetics, metabolism Mutation Protein-Tyrosine Kinases / genetics, metabolism Rad52 DNA Repair and Recombination Protein / genetics*, metabolism Transcription Factors / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
1 R01 AI51949/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/CPH1 protein, Candida albicans; 0/Cell Cycle Proteins; 0/Cyclins; 0/DNA-Binding Proteins; 0/EFG1 protein, Candida albicans; 0/Fungal Proteins; 0/HWP1 protein, Candida albicans; 0/Membrane Glycoproteins; 0/Rad52 DNA Repair and Recombination Protein; 0/Transcription Factors; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 4.6.1.1/Adenylate Cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: CRZ1, a target of the calcineurin pathway in Candida albicans.
Next Document: Borrelia burgdorferi lipoproteins are secreted to the outer surface by default.