| Rac1, and not Rac2, is involved in the regulation of the intracellular hydrogen peroxide level in HepG2 cells. | |
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MedLine Citation:
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PMID: 9576845 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to elucidate the components of the oxygen sensory complex in HepG2 cells which regulates the production of erythropoietin, we have microinjected recombinant variants of the human small GTP-binding protein hRac1 and measured their effects on the production of reactive oxygen species (ROS) by the dihydrorhodamine-123 technique. The dominant-negative mutant hRac1(T17N) inhibits the NADH-stimulated production of ROS in HepG2 cells, whereas the constitutively activated hRac1(G12V) leads to an increase in intracellular ROS concentration. Reverse transcriptase PCR analysis showed that the hRac1, but not the hRac2, gene is expressed in HepG2 cells. These results demonstrate that hRac1, and not hRac2, is involved in the regulation of ROS production in HepG2 cells and suggest that hRac1 specifically functions in the non-phagocytic NAD(P)H oxidase complex. |
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Authors:
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R H Cool; E Merten; C Theiss; H Acker |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 332 ( Pt 1) ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1998 May |
Date Detail:
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Created Date: 1998-08-17 Completed Date: 1998-08-17 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 5-8 Citation Subset: IM |
Affiliation:
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Max-Planck-Institut für Molekulare Physiologie, Abteilung Strukturelle Biologie, Rheinlanddam 201, D-44139 Dortmund, Postfach 102664, D-44026 Dortmund, Germany. robbert.cool@mpi-dortmund.mpg.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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GTP-Binding Proteins
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genetics,
physiology* Gene Expression Regulation, Neoplastic Humans Hydrogen Peroxide / metabolism* Lymphocytes / metabolism Microinjections NAD / pharmacology NADH, NADPH Oxidoreductases / metabolism Neoplasm Proteins / physiology Polymerase Chain Reaction RNA, Messenger / analysis Reactive Oxygen Species / metabolism Recombinant Proteins / metabolism Tumor Cells, Cultured rac GTP-Binding Proteins |
| Chemical | |
Reg. No./Substance:
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0/Neoplasm Proteins; 0/RNA, Messenger; 0/Reactive Oxygen Species; 0/Recombinant Proteins; 53-84-9/NAD; 7722-84-1/Hydrogen Peroxide; EC 1.6.-/NADH, NADPH Oxidoreductases; EC 3.6.1.-/GTP-Binding Proteins; EC 3.6.5.2/rac GTP-Binding Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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