Document Detail


Rac1 modulates the vitreous-induced plasticity of mesenchymal movement in retinal pigment epithelial cells.
MedLine Citation:
PMID:  23331298     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The vitreous has been shown to induce epithelial-mesenchymal transdifferentiation (EMT) because it induces fibroblast-like morphology, enhanced migration and invasion in retinal pigment epithelial (RPE) cells in proliferative vitreoretinopathy (PVR). Rac1 is the principal mediator of cell migration. In the current study, the relationship between Rac1 and cell migration and invasion in vitreous-transformed RPE cells was investigated using NSC23766, a specific inhibitor of Rac GTPase activity, and the involvement of a Rac1 GTPase-dependent pathway was detected. METHODS: Human low-passage RPE cells were treated with normal medium or 25% vitreous medium. Rac1 activity was measured using a pulldown assay. The cytotoxicity of NSC23766 was measured using the trypan blue dye exclusion test. Cell migration was measured using a wound healing assay. Cell invasion was determined using a transwell invasion assay. Protein expression of Rac1 and phosphorylation of LIMK1 and cofilin were detected by Western blot analysis. RESULTS: Rac1 GTPase was activated in vitreous-transformed RPE cells. A Rac inhibitor suppressed vitreous-induced migration and invasion in RPE cells. Cofilin phosphorylation was activated by vitreous treatment but blocked by NSC23766. CONCLUSIONS: Rac1 mediates vitreous-transformed RPE cells' plasticity of mesenchymal movement via Rac1 GTPase-dependent pathways that modulate LIMK1 and cofilin activity. Rac inhibition may be considered a novel treatment for PVR.
Authors:
Xiong-Gao Huang; You-Zheng Chen; Zhao-Tian Zhang; Yan-Tao Wei; Hai-Zhi Ma; Ting Zhang; Shao-Chong Zhang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-21
Journal Detail:
Title:  Clinical & experimental ophthalmology     Volume:  -     ISSN:  1442-9071     ISO Abbreviation:  Clin. Experiment. Ophthalmol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100896531     Medline TA:  Clin Experiment Ophthalmol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors. Clinical and Experimental Ophthalmology © 2013 Royal Australian and New Zealand College of Ophthalmologists.
Affiliation:
State Key Ophthalmic Laboratory, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China; Hainan Eye Hospital, Zhongshan Ophthalmic Center, Sun Yat-sen University, Haikou, China.
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