Document Detail

Rac1 and RhoA GTPases have antagonistic functions during N-cadherin-dependent cell-cell contact formation in C2C12 myoblasts.
MedLine Citation:
PMID:  17459003     Owner:  NLM     Status:  MEDLINE    
BACKGROUND INFORMATION: N-cadherin, a member of the Ca(2+)-dependent cell-cell adhesion molecule family, plays an essential role in the induction of the skeletal muscle differentiation programme. However, the molecular mechanisms which govern the formation of N-cadherin-dependent cell-cell contacts in myoblasts remain unexplored. RESULTS: In the present study, we show that N-cadherin-dependent cell contact formation in myoblasts is defined by two stages. In the first phase, N-cadherin is highly mobile in the lamellipodia extensions between the contacting cells. The second stage corresponds to the formation of mature N-cadherin-dependent cell contacts, characterized by the immobilization of a pool of N-cadherin which appears to be clustered in the interdigitated membrane structures that are also membrane attachment sites for F-actin filaments. We also demonstrated that the formation of N-cadherin-dependent cell-cell contacts requires a co-ordinated and sequential activity of Rac1 and RhoA. Rac1 is involved in the first stage and facilitates N-cadherin-dependent cell-cell contact formation, but it is not absolutely required. Conversely, RhoA is necessary for N-cadherin-dependent cell contact formation, since, via ROCK (Rho-associated kinase) signalling and myosin 2 activation, it allows the stabilization of N-cadherin at the cell-cell contact sites. CONCLUSIONS: We have shown that Rac1 and RhoA have opposite effects on N-cadherin-dependent cell-cell contact formation in C2C12 myoblasts and act sequentially to allow its formation.
Franck Comunale; Marie Causeret; Cyril Favard; Julien Cau; Nicolas Taulet; Sophie Charrasse; Cécile Gauthier-Rouvière
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of the cell / under the auspices of the European Cell Biology Organization     Volume:  99     ISSN:  1768-322X     ISO Abbreviation:  Biol. Cell     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-16     Completed Date:  2007-10-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8108529     Medline TA:  Biol Cell     Country:  England    
Other Details:
Languages:  eng     Pagination:  503-17     Citation Subset:  IM    
CRBM, CNRS, 1919 Route de Mende, 34293 Montpellier, France.
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MeSH Terms
Cadherins / drug effects,  metabolism*
Cell Adhesion / drug effects,  physiology
Cells, Cultured
Myoblasts / metabolism*
rac1 GTP-Binding Protein / physiology*
rhoA GTP-Binding Protein / physiology*
Reg. No./Substance:
0/Cadherins; 0/RAC1 protein, human; EC GTP-Binding Protein; EC GTP-Binding Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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