| Rac1 and RhoA GTPases have antagonistic functions during N-cadherin-dependent cell-cell contact formation in C2C12 myoblasts. | |
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MedLine Citation:
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PMID: 17459003 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND INFORMATION: N-cadherin, a member of the Ca(2+)-dependent cell-cell adhesion molecule family, plays an essential role in the induction of the skeletal muscle differentiation programme. However, the molecular mechanisms which govern the formation of N-cadherin-dependent cell-cell contacts in myoblasts remain unexplored. RESULTS: In the present study, we show that N-cadherin-dependent cell contact formation in myoblasts is defined by two stages. In the first phase, N-cadherin is highly mobile in the lamellipodia extensions between the contacting cells. The second stage corresponds to the formation of mature N-cadherin-dependent cell contacts, characterized by the immobilization of a pool of N-cadherin which appears to be clustered in the interdigitated membrane structures that are also membrane attachment sites for F-actin filaments. We also demonstrated that the formation of N-cadherin-dependent cell-cell contacts requires a co-ordinated and sequential activity of Rac1 and RhoA. Rac1 is involved in the first stage and facilitates N-cadherin-dependent cell-cell contact formation, but it is not absolutely required. Conversely, RhoA is necessary for N-cadherin-dependent cell contact formation, since, via ROCK (Rho-associated kinase) signalling and myosin 2 activation, it allows the stabilization of N-cadherin at the cell-cell contact sites. CONCLUSIONS: We have shown that Rac1 and RhoA have opposite effects on N-cadherin-dependent cell-cell contact formation in C2C12 myoblasts and act sequentially to allow its formation. |
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Authors:
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Franck Comunale; Marie Causeret; Cyril Favard; Julien Cau; Nicolas Taulet; Sophie Charrasse; Cécile Gauthier-Rouvière |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biology of the cell / under the auspices of the European Cell Biology Organization Volume: 99 ISSN: 1768-322X ISO Abbreviation: Biol. Cell Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2007-08-16 Completed Date: 2007-10-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8108529 Medline TA: Biol Cell Country: England |
Other Details:
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Languages: eng Pagination: 503-17 Citation Subset: IM |
Affiliation:
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CRBM, CNRS, 1919 Route de Mende, 34293 Montpellier, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cadherins
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drug effects,
metabolism* Cell Adhesion / drug effects, physiology Cells, Cultured Humans Myoblasts / metabolism* rac1 GTP-Binding Protein / physiology* rhoA GTP-Binding Protein / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Cadherins; 0/RAC1 protein, human; EC 3.6.5.2/rac1 GTP-Binding Protein; EC 3.6.5.2/rhoA GTP-Binding Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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