Document Detail


Rabbit tonsil-associated M-cells express cytokeratin 20 and take up particulate antigen.
MedLine Citation:
PMID:  15385578     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
M-cells are believed to play a pivotal role in initiation of the immune response. These cells, located in the epithelia that overlie mucosal lymphoid follicles, are responsible for the active uptake of particulate antigens and for their translocation to the underlying lymphoid tissue. The identification of reliable markers for M-cells is therefore extremely important for the study of the initial steps that lead to the immune response. For this purpose, we studied cytokeratin 20 (CK20) expression in the epithelium of rabbit palatine tonsils by immunofluorescence, confocal microscopy, and Western blotting. CK20+ cells were observed in all rabbit palatine tonsils examined. By Western blotting, one CK20-immunoreactive band was identified at 46 kD on samples of proteins from the intermediate filament-enriched cytoskeletal fraction of tonsil epithelium. Double labeling of CK20+ cells with cell-specific markers confirmed that such cells were actually M-cells. Moreover, CK20+ M-cells displayed a mature phenotype (they formed pockets harboring lymphoid cells) and were functionally competent because they could take up particulate antigens from the pharyngeal lumen. We conclude that CK20 is an M-cell marker for rabbit palatine tonsils. Moreover, we can hypothesize the use of M-cells as a possible site for antigen delivery of particle-based vaccines.
Authors:
Alessandro Carapelli; Marì Regoli; Claudio Nicoletti; Leonardo Ermini; Luciano Fonzi; Eugenio Bertelli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  52     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-09-23     Completed Date:  2004-11-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1323-32     Citation Subset:  IM    
Copyright Information:
Copyright The Histochemical Society, Inc.
Affiliation:
Dept. of Pharmacology "Giorgio Segre," Section of Morphology, Via Aldo Moro 4, University of Siena, Siena, Italy. bertelli5@unisi.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Fluorescent Antibody Technique
Fluorescent Dyes / chemistry
Intermediate Filament Proteins / biosynthesis*
Keratin-20
Male
Microscopy, Confocal
Microspheres
Palatine Tonsil / cytology,  metabolism*
Rabbits
Respiratory Mucosa / cytology,  metabolism
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Intermediate Filament Proteins; 0/Keratin-20

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