Document Detail

Rabbit heart can be "preconditioned" via transfer of coronary effluent.
MedLine Citation:
PMID:  10600868     Owner:  NLM     Status:  MEDLINE    
Brief myocardial ischemia not only evokes a local cardioprotective or "preconditioning" effect but also can render remote myocardium resistant to sustained ischemia. We propose the following hypotheses: remote protection is initiated by a humoral trigger; brief ischemia-reperfusion will result in release of the humoral trigger (possibly adenosine and/or norepinephrine) into the coronary effluent; and transfer of this effluent to a virgin acceptor heart will elicit cardioprotection. To test these concepts, effluent was collected during normal perfusion from donor-control hearts and during preconditioning ischemia-reperfusion from donor-preconditioned (PC) hearts. After reoxygenation occurred and aliquots for measurement of adenosine and norepinephrine content were harvested, effluent was transfused to acceptor-control and acceptor-PC hearts. All hearts then underwent 40 min of global ischemia and 60 min of reperfusion, and infarct size was delineated by tetrazolium staining. Mean infarct size was smaller in both donor- and acceptor-PC groups (9% of left ventricle) than in donor- and acceptor-control groups (36% and 34%; P < 0.01). Protection in acceptor-PC hearts could not, however, be attributed to adenosine or norepinephrine. Thus preconditioning-induced cardioprotection can be transferred between rabbit hearts by transfusion of coronary effluent. Although adenosine and norepinephrine are apparently not responsible, these results suggest that remote protection is initiated by a humoral mechanism.
E W Dickson; M Lorbar; W A Porcaro; R A Fenton; C P Reinhardt; A Gysembergh; K Przyklenk
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The American journal of physiology     Volume:  277     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-01-19     Completed Date:  2000-01-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H2451-7     Citation Subset:  IM    
Department of Emergency Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA.
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MeSH Terms
Adenosine / blood*
Blood Transfusion*
Coronary Circulation
Coronary Vessels / physiology*
Ischemic Preconditioning / methods*
Models, Cardiovascular
Myocardial Infarction / pathology,  physiopathology*,  prevention & control
Myocardial Ischemia / physiopathology*
Myocardial Reperfusion
Norepinephrine / blood*
Time Factors
Ventricular Function, Left
Reg. No./Substance:
51-41-2/Norepinephrine; 58-61-7/Adenosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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