Document Detail

Rabbit ear model of injury-induced arterial smooth muscle cell proliferation. Kinetics, reproducibility, and implications.
MedLine Citation:
PMID:  1873869     Owner:  NLM     Status:  MEDLINE    
Recently, considerable interest has focused on the vascular smooth muscle cell (SMC) response to injury, particularly as it relates to restenosis after angioplasty. In an effort to find an optimal experimental model of arterial SMC proliferation after injury, we examined the effects of external injury to the central artery of the rabbit ear and assessed the reproducibility, morphological changes, and time course of cellular proliferation after such an injury. With rabbits under general anesthesia, direct pressure was applied at two sites along the central artery of the ears of 19 New Zealand White rabbits. Rabbits were maintained on a diet of 2.4% fat and 0.001% cholesterol throughout the experiment. In seven rabbits examined after 21 days, marked SMC proliferation with neointimal formation was observed at all 28 sites (100%). Mean neointimal area, expressed as a percentage of the area of the tunica media, was 82 +/- 40% (range, 21-203%). Compared with the uninvolved artery displaced 2 mm from the injury site, mechanical crush caused a 38% increase in total vessel area (p less than 0.001), a 40% decrease in luminal area (p less than 0.002), and no change in the area of the media. Serial histological studies were performed 1-42 days after injury, using light and electron microscopy and bromodeoxyuridine immunohistochemistry. Beginning at day 3, activated medial SMCs were noted to migrate through defects in the internal elastic membrane, with a gradual increase in neointimal area between days 5 and 12. Peak DNA synthesis was identified in the media 5 days after injury, with proliferative activity shifting almost exclusively to the neointima thereafter.(ABSTRACT TRUNCATED AT 250 WORDS)
S Banai; M Shou; R Correa; M T Jaklitsch; P C Douek; R F Bonner; S E Epstein; E F Unger
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Circulation research     Volume:  69     ISSN:  0009-7330     ISO Abbreviation:  Circ. Res.     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-09-26     Completed Date:  1991-09-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  748-56     Citation Subset:  IM    
Laboratory of Experimental Physiology and Pharmacology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md 20892.
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MeSH Terms
Arteries / injuries*
Arteriosclerosis / etiology
Cell Division
Cytoplasm / ultrastructure
Ear / blood supply
Endoplasmic Reticulum / ultrastructure
Mitochondrial Swelling
Muscle, Smooth, Vascular / cytology,  injuries*,  ultrastructure
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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