Document Detail


Rab5 GTPase controls chromosome alignment through Lamin disassembly and relocation of the NuMA-like protein Mud to the poles during mitosis.
MedLine Citation:
PMID:  21987826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The small GTPase Rab5 is a conserved regulator of membrane trafficking; it regulates the formation of early endosomes, their transport along microtubules, and the fusion to the target organelles. Although several members of the endocytic pathway were recently implicated in spindle organization, it is unclear whether Rab5 has any role during mitosis. Here, we describe that Rab5 is required for proper chromosome alignment during Drosophila mitoses. We also found that Rab5 associated in vivo with nuclear Lamin and mushroom body defect (Mud), the Drosophila counterpart of nuclear mitotic apparatus protein (NuMA). Consistent with this finding, Rab5 was required for the disassembly of the nuclear envelope at mitotic entry and the accumulation of Mud at the spindle poles. Furthermore, Mud depletion caused chromosome misalignment defects that resembled the defects of Rab5 RNAi cells, and double-knockdown experiments indicated that the two proteins function in a linear pathway. Our results indicate a role for Rab5 in mitosis and reinforce the emerging view of the contributions made by cell membrane dynamics to spindle function.
Authors:
Luisa Capalbo; Pier Paolo D'Avino; Vincent Archambault; David M Glover
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-10-10
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  108     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-19     Completed Date:  2011-12-30     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  17343-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Chromosome Segregation / physiology*
Drosophila Proteins / metabolism*
Green Fluorescent Proteins / metabolism
Kinetochores / metabolism
Lamins / metabolism*
Membrane Proteins / metabolism*
Microtubules / metabolism
Mitosis / physiology
Nerve Tissue Proteins / metabolism*
Protein-Serine-Threonine Kinases / metabolism
RNA Interference
Recombinant Fusion Proteins / metabolism
Spindle Apparatus / metabolism
rab5 GTP-Binding Proteins / antagonists & inhibitors,  genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
11431//Cancer Research UK; G1001696//Medical Research Council; //Biotechnology and Biological Sciences Research Council; //Cancer Research UK
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Lamin protein, Drosophila; 0/Lamins; 0/Membrane Proteins; 0/Mud protein, Drosophila; 0/Nerve Tissue Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; EC 2.7.11.-/polo protein, Drosophila; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 3.6.5.2/rab5 GTP-Binding Proteins
Comments/Corrections

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