| Rab conversion as a mechanism of progression from early to late endosomes. | |
| | |
MedLine Citation:
|
PMID: 16143105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The mechanisms of endosome biogenesis and maintenance are largely unknown. The small GTPases Rab 5 and Rab 7 are key determinants of early and late endosomes, organizing effector proteins into specific membrane subdomains. Whether such Rab machineries are indefinitely maintained on membranes or can disassemble in the course of cargo transport is an open question. Here, we combined novel image-analysis algorithms with fast live-cell imaging. We found that the level of Rab 5 dynamically fluctuates on individual early endosomes, linked by fusion and fission events into a network in time. Within it, degradative cargo concentrates in progressively fewer and larger endosomes that migrate from the cell periphery to the center where Rab 5 is rapidly replaced with Rab 7. The class C VPS/HOPS complex, an established GEF for Rab 7, interacts with Rab 5 and is required for Rab 5-to-Rab 7 conversion. Our results reveal unexpected dynamics of Rab domains and suggest Rab conversion as the mechanism of cargo progression between early and late endosomes. |
| | |
Authors:
|
Jochen Rink; Eric Ghigo; Yannis Kalaidzidis; Marino Zerial |
Related Documents
:
|
18379325 - Pilomatricoma: magnetic resonance imaging and pathological evaluation. 17882025 - Usefulness of fat-suppressed t1-weighted mri using orally administered superparamagneti... 15990015 - Assessment of the tumor microenvironment in cervix cancer using dynamic contrast enhanc... 1535875 - Carcinoma of the cervix: dynamic mr imaging. 12014875 - Unexpected subdiaphragmatic findings on ct of the chest in septic patients after cardia... 12915195 - Quantitative assessment of gd-dtpa contrast agent from signal enhancement: an in-vitro ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Cell Volume: 122 ISSN: 0092-8674 ISO Abbreviation: Cell Publication Date: 2005 Sep |
Date Detail:
|
Created Date: 2005-09-06 Completed Date: 2005-11-08 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0413066 Medline TA: Cell Country: United States |
Other Details:
|
Languages: eng Pagination: 735-49 Citation Subset: IM |
Affiliation:
|
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biological Transport Cell Line, Tumor Endosomes / metabolism* Fluorescent Dyes Humans Image Processing, Computer-Assisted Lipoproteins, LDL / metabolism Microscopy, Video Models, Biological rab GTP-Binding Proteins / metabolism* rab5 GTP-Binding Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Fluorescent Dyes; 0/Lipoproteins, LDL; 152989-05-4/rab7 protein; EC 3.6.1.-/rab GTP-Binding Proteins; EC 3.6.5.2/rab5 GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The Set1 methyltransferase opposes Ipl1 aurora kinase functions in chromosome segregation.
Next Document: Role of endocytosis in cellular uptake of sex steroids.