Document Detail


RSR13, a potential athletic performance enhancement agent: detection in urine by gas chromatography/mass spectrometry.
MedLine Citation:
PMID:  11746905     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RSR13 (2-[4-[[(3,5-dimethylanilino)carbonyl]methyl]phenoxyl]-2-methylpropionic acid) is a synthetic allosteric modifier of hemoglobin that is currently in a phase III clinical trial as a radio-enhancing agent. RSR13 has been shown to increase maximum oxygen uptake (VO(2max)) in a canine skeletal model, which makes it a potential performance-enhancing agent for endurance athletes, since VO(2max) is an index of aerobic capacity. In this study we present a method for the detection of RSR13-bis-TMS in human urine by gas chromatography/electron impact ionization mass spectrometry (GC/EI-MS) suitable for doping control laboratories. The presence of RSR13 is detected by monitoring the ions m/z 485 ([M](+.)) and 470 ([M - CH3](+)). The limit of detection (LOD) is less than 2 ng/mL in urine. Urine samples collected from clinical trial subjects immediately prior to receiving an infusion of RSR13 showed no evidence of RSR13, whereas post-infusion urine samples contained up to 1181 microg/mL. A urine sample collected 36 h after administration of a small dose (10 mg/kg) and diluted 100-fold showed a signal 80 times higher than the LOD. Urine samples obtained from 100 randomly selected athletes in our routine testing program did not show any traces of RSR13. Sport authorities may wish to add RSR13 to the list of prohibited substances.
Authors:
A Breidbach; D H Catlin
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  15     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2001  
Date Detail:
Created Date:  2001-12-17     Completed Date:  2002-03-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  2379-82     Citation Subset:  IM    
Copyright Information:
Copyright 2001 John Wiley & Sons, Ltd.
Affiliation:
UCLA Olympic Analytical Laboratory, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90025, USA.
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MeSH Terms
Descriptor/Qualifier:
Aniline Compounds / urine*
Calibration
Chromatography, Gas
Doping in Sports*
Glucuronides / urine
Humans
Indicators and Reagents
Propionic Acids / urine*
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization
Substance Abuse Detection / methods*
Chemical
Reg. No./Substance:
0/Aniline Compounds; 0/Glucuronides; 0/Indicators and Reagents; 0/Propionic Acids; 131179-95-8/efaproxiral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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