Document Detail

RP-HPLC determination of water-soluble vitamins in honey.
MedLine Citation:
PMID:  21147338     Owner:  NLM     Status:  In-Data-Review    
The assessment and validation of reliable analytical methods for the determination of vitamins in sugar-based matrices (e.g. honey) are still scarcely explored fields of research. This study proposes and fully validates a simple and fast RP-HPLC method for the simultaneous determination of five water-soluble vitamins (vitamin B(2), riboflavin; vitamin B(3), nicotinic acid; vitamin B(5), pantothenic acid; vitamin B(9), folic acid; and vitamin C, ascorbic acid) in honey. The method provides low detection and quantification limits, very good linearity in a large concentration interval, very good precision, and the absence of any bias. It has been successfully applied to 28 honey samples (mainly from Sardinia, Italy) of 12 different botanical origins. While the overall amount of the analytes in the samples is quite low (always below 40mgkg(-1)), we have observed a marked dependence of some of their concentrations (i.e. vitamin B(3) and vitamin B(5)) and the botanical origin of the honey. This insight might lead to important characterization features for this food item.
Marco Ciulu; Silvia Solinas; Ignazio Floris; Angelo Panzanelli; Maria I Pilo; Paola C Piu; Nadia Spano; Gavino Sanna
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Publication Detail:
Type:  Journal Article     Date:  2010-11-04
Journal Detail:
Title:  Talanta     Volume:  83     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  924-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Università degli Studi di Sassari, Dipartimento di Chimica, via Vienna 2, 07100 Sassari, Italy.
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