Document Detail


ROS-mediated autophagy was involved in cancer cell death induced by novel copper(II) complex.
MedLine Citation:
PMID:  19758794     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we investigated autophagy induced in HeLa cells by copper(II) complex of ethyl 2-[bis(2-pyridylmethyl)amino] propionate ligand (ETDPA) (formula: [(ETDPA)Cu(phen)](ClO4)2 (abbreviated as LCu),a novel synthetic copper(II) complex whose DNA binding activity has been proved. Cell viability, autophagic levels and generation of ROS were evaluated following the exposure to LCu. LCu-induced cell death in a dose- and time-dependent manner, which was demonstrated by enhanced fluorescence intensity of monodansylcadervarine (MDC), as well as elevated expression of autophagy-related protein MAP-LC3. These phenomena were all attenuated after pretreatment with autophagy inhibitors 3-MA or NH(4)Cl. Furthermore, our data indicated that LCu-triggered autophagy through ROS: cellular ROS levels were increased after LCu treatment, which was reversed by ROS scavenger NAC (N-acetylcysteine). As a consequence, Lcu-mediated autophagy was partly blocked by NAC. In summary, we synthesized a novel copper(II) complex and showed that this compound was effective in killing HeLa cells via ROS-triggered autophagic pathway.
Authors:
Wen-jie Guo; Si-si Ye; Ning Cao; Juan Huang; Jing Gao; Qiu-yun Chen
Related Documents :
20107314 - Osu-03012 enhances ad.7-induced gbm cell killing via er stress and autophagy and by dec...
16757804 - Bax inhibitor-1 protects neurons from oxygen-glucose deprivation.
12668724 - Oxidant-induced cell death in retinal pigment epithelium cells mediated through the rel...
19373244 - Rocaglamide sensitizes leukemic t cells to activation-induced cell death by differentia...
8555374 - Effect of melphalan and hyperthermia on cell cycle progression and cyclin b1 expression...
12876074 - Melanotrope secretory cycle is regulated by physiological inputs via the hypothalamus.
Publication Detail:
Type:  Journal Article     Date:  2009-09-16
Journal Detail:
Title:  Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie     Volume:  62     ISSN:  1618-1433     ISO Abbreviation:  Exp. Toxicol. Pathol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-09     Completed Date:  2010-11-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208920     Medline TA:  Exp Toxicol Pathol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  577-82     Citation Subset:  IM    
Copyright Information:
(c) 2009 Elsevier GmbH. All rights reserved.
Affiliation:
School of Pharmacy, Jiangsu University, Zhenjiang 212013, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology*
Autophagy / drug effects*
Blotting, Western
Cell Proliferation / drug effects*
Coordination Complexes / pharmacology*
Hela Cells
Humans
Organometallic Compounds / pharmacology*
Reactive Oxygen Species / metabolism*
Chemical
Reg. No./Substance:
0/(Cu(4-methylcoumarin-6,7-dioxactetate)(phenanthroline)2); 0/Antineoplastic Agents; 0/Coordination Complexes; 0/Organometallic Compounds; 0/Reactive Oxygen Species

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  G-protein-coupled receptor 40 (GPR40) expression and its regulation in human pancreatic islets: The ...
Next Document:  Effects of total glucosides of paeony on oxidative stress in the kidney from diabetic rats.