Document Detail

ROS-mediated autophagy was involved in cancer cell death induced by novel copper(II) complex.
MedLine Citation:
PMID:  19758794     Owner:  NLM     Status:  MEDLINE    
In this study, we investigated autophagy induced in HeLa cells by copper(II) complex of ethyl 2-[bis(2-pyridylmethyl)amino] propionate ligand (ETDPA) (formula: [(ETDPA)Cu(phen)](ClO4)2 (abbreviated as LCu),a novel synthetic copper(II) complex whose DNA binding activity has been proved. Cell viability, autophagic levels and generation of ROS were evaluated following the exposure to LCu. LCu-induced cell death in a dose- and time-dependent manner, which was demonstrated by enhanced fluorescence intensity of monodansylcadervarine (MDC), as well as elevated expression of autophagy-related protein MAP-LC3. These phenomena were all attenuated after pretreatment with autophagy inhibitors 3-MA or NH(4)Cl. Furthermore, our data indicated that LCu-triggered autophagy through ROS: cellular ROS levels were increased after LCu treatment, which was reversed by ROS scavenger NAC (N-acetylcysteine). As a consequence, Lcu-mediated autophagy was partly blocked by NAC. In summary, we synthesized a novel copper(II) complex and showed that this compound was effective in killing HeLa cells via ROS-triggered autophagic pathway.
Wen-jie Guo; Si-si Ye; Ning Cao; Juan Huang; Jing Gao; Qiu-yun Chen
Related Documents :
16466914 - Progestin inhibition of cell death in human breast cancer cell lines.
11393284 - Herpes simplex virus thymidine kinase/ganciclovir-induced cell death is enhanced by co-...
17180044 - The rodent non-genotoxic hepatocarcinogen nafenopin and egf alter the mitosis/apoptosis...
20025854 - Neuroprotective effects of (-)-epigallocatechin-3-gallate against quinolinic acid-induc...
19448034 - The organization pattern of root border-like cells of arabidopsis is dependent on cell ...
12226744 - A key role of the hsnf5/ini1 tumour suppressor in the control of the g1-s transition of...
Publication Detail:
Type:  Journal Article     Date:  2009-09-16
Journal Detail:
Title:  Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie     Volume:  62     ISSN:  1618-1433     ISO Abbreviation:  Exp. Toxicol. Pathol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-09     Completed Date:  2010-11-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208920     Medline TA:  Exp Toxicol Pathol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  577-82     Citation Subset:  IM    
Copyright Information:
(c) 2009 Elsevier GmbH. All rights reserved.
School of Pharmacy, Jiangsu University, Zhenjiang 212013, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antineoplastic Agents / pharmacology*
Autophagy / drug effects*
Blotting, Western
Cell Proliferation / drug effects*
Coordination Complexes / pharmacology*
Hela Cells
Organometallic Compounds / pharmacology*
Reactive Oxygen Species / metabolism*
Reg. No./Substance:
0/(Cu(4-methylcoumarin-6,7-dioxactetate)(phenanthroline)2); 0/Antineoplastic Agents; 0/Coordination Complexes; 0/Organometallic Compounds; 0/Reactive Oxygen Species

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  G-protein-coupled receptor 40 (GPR40) expression and its regulation in human pancreatic islets: The ...
Next Document:  Effects of total glucosides of paeony on oxidative stress in the kidney from diabetic rats.